Endogenous cellulolytic enzyme systems in the longhorn beetle Mesosa myops （Insecta： Coleoptera） studied by transcriptomic analysis

• Published in: 生物化学与生物物理学报：英文版 2015 (9), p.741-748
• Main Author: Jie Liu Keqing Song Huajing Teng Bin Zhang Wenzhu Li Huaijun Xue Xingke Yang
• Format: Article
• Language: English
• Subjects: 内源性; 天牛; 昆虫纲; 糖苷水解酶; 纤维素酶基因; 组分; 酶系统; 鞘翅目
• ISSN: 1672-9145; 1745-7270

The Cerambycidae （longhorn beetle） is a large family of Coleoptera with xylophagous feeding habits. Cellulose digestion plays an important role in these wood-feeding insects. In this study, transcrip- tomic technology was used to obtain one glycoside hydrolase family 45 （GH45） cellulase and seven GH5 cellulases from Mesosa myops, a typical longhorn beetle. Analyses of expression dynam- ics and evolutionary relationships provided a complete description of the cellulolytic system. The expression dynamics related to individual development indicated that endogenous GH45 and GH5 cellulases dominate cellulose digestion in M. myops. Evolutionary analyses suggested that GH45 cellulase gene is a general gene in the CoLeoptera Suborder Polyphaga, Evolutionary analyses also indicated that the GH5 cellulase group in Lamiinae longhorn beetles is closely associated with wood feeding. This study demonstrated that there is a complex endogenous cellulolytic system in M. myops that is dominated by cellulases belonging to two glycoside hydrolase families.