Duplex PCR Detection of Pseudomonas savastanoi pv. phaseolicola and Curtobacterium flaccumfaciens pv, flaccumfaciens in Soybean

The paper aimed to establish a duplex PCR method for simultaneous detection of Pseudomonas savastanoi pv. Phaseolicola （Psp） and Curtobaeterium /accumfadens pv. Flaccumfaciens （Cff）. Based on the argK gene of Psp in GenBank, the primers PSPF1/PSPR2 were designed. The duplex PCR assay was dereloped u...

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Published in:Plant Diseases and Pests(植物病虫害研究：英文版) 2016, Vol.7 (1), p.27-31
Main Author: Liu Yan Yang Wanfeng Liu Xiang Shao Peize Chen Yunqing Zhao Wenjun
Format: Article
Language:English
Subjects:
GenBank
PCR方法
PCR检测
基因组DNA
菜豆
萎蔫短小杆菌
进口大豆
铜绿假单胞菌
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Summary:The paper aimed to establish a duplex PCR method for simultaneous detection of Pseudomonas savastanoi pv. Phaseolicola （Psp） and Curtobaeterium /accumfadens pv. Flaccumfaciens （Cff）. Based on the argK gene of Psp in GenBank, the primers PSPF1/PSPR2 were designed. The duplex PCR assay was dereloped using the combined primers PSPF1/PSPR2 and CflF1/CffR2, which were specific primers for Cff. The reaction conditions were optimized and specificity md sensitivity of the duplex PCR were tested. The expected DNA fragment was specifically amplified from the genomic DNA of Psp and Cff. Specificity was conirmed in the artificially inoculated soybean samples imparted. Thus, the duplex PCR developed in this study could be used for the simultaneous detection of Psp md Cff from imported soybean.
ISSN:2152-3932