Superresolution imaging of telomeres with continuous wave stimulated emission depletion （STED） microscope

The significant role of telomeres in cells has attracted much attention since they were discovered.Fluorescence imaging is an effective method to study subcellular structures like telomeres.However,the diffraction limit of traditional optical microscope hampers further investigation on them.Recent p...

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Bibliographic Details
Published in:中国科学：化学英文版 2016 (11), p.1519-1524
Main Author: Shaopeng Wang Suhui Deng Xiaoqing Cai Shangguo Hou Jiajun Li Zhaoshuai Gao Jiang Li Lihua Wang Chunhai Fan
Format: Article
Language:English
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Summary:The significant role of telomeres in cells has attracted much attention since they were discovered.Fluorescence imaging is an effective method to study subcellular structures like telomeres.However,the diffraction limit of traditional optical microscope hampers further investigation on them.Recent progress on superresolution fluorescence microscopy has broken this limit.In this work,we used stimulated emission depletion（STED） microscope to observe fluorescence-labeled telomeres in interphase cell nuclei.The results showed that the size of fluorescent puncta representing telomeres under the STED microscope was much smaller than that under the confocal microscope.Two adjacent telomeres were clearly separated via STED imaging,which could hardly be discriminated by confocal microscopy due to the diffraction limit.We conclude that STED microscope is a more powerful tool that enable us to obtain detailed information about telomeres.
ISSN:1674-7291
1869-1870