Effects of Shenfu Injection （参附注射液） on Cerebral Metabolism in A Porcine Model of Cardiac Arrest

• Published in: 中国结合医学杂志：英文版 2017, Vol.23 (1), p.33-39
• Main Author: YIN Qin WU Cai-jun YANG Jun HANG Chen-chen LI Chun-sheng
• Format: Article
• Language: English
• Subjects: 代谢; 模型; 注射液; 猪心脏; 神经功能; 组织病理学; 肾上腺素; 脑脊液
• ISSN: 1672-0415; 1993-0402

Objective： To investigate the effects of Shenfu Injection （参附注射液, SFI） on cerebral metabolism in a porcine model of cardiac arrest （CA）. Methods： Thirty Wuzhishan minipigs were randomly assigned to the control group （n=6）, epinephrine group （Fp group, n=12） and SFI group （n=12）. After 8 min of untreated ventricular fibrillation （VF）, pigs in the EP group or SFI group were administered with either i=p （0.02 mg/kg） or SFI （1.0 mL/kg）, respectively. After successful resuscitation, cerebrospinal fluid （CSF） levels of glucose, pyruvate, lactate, glutamate and glycerol were measured at 1, 6, 12 and 24 h after recover from spontaneous circulation （ROSC）. In addition, neurologic deficit score （NDS） was calculated at 24 h after ROSC. Surviving pigs were killed at 24 h after ROSC, and the brain tissue was obtained for ultra-microstructure examination. Results： Compared with the EP group, CSF glucose and pyruvate levels were higher （all P〈0.01）, and lactate levels were lower in the SFI group （P〈0.01）. Meanwhile, CSF glutamate and glycerol levels in the SFI group were lower in comparison to the EP group （all P〈0.05）. In addition, SFI decreased NDS at 24 h after ROSC （P〈0.01）, and alleviated the histopathological damage of the brain. Conclusions： SFI could alleviate brain injury after CA, which may be associated with improving cerebral metabolism.