Antioxidant mediated response noise-induced redox imbalance changes in rat brain of Scoparia dulcis in and immunohistochemical

• Published in: 生物医学研究杂志：英文版 2017, Vol.31 (2), p.143-153
• Main Author: Wankupar Wankhar Sakthivel Srinivasan Ravindran Rajan Rathinasamy Sheeladevi
• Format: Article
• Language: English
• Subjects: 介导; 免疫组化; 内源性自由基; 噪声暴露; 大鼠脑; 抗氧化剂; 抗氧化活性; 氧化还原平衡
• ISSN: 1674-8301

Noise has been regarded as an environmental/occupational stressor that causes damages to both auditory and non- auditory organs. Prolonged exposure to these mediators of stress has often resulted in detrimental effect, where oxidative/nitrosative stress plays a major role. Hence, it would be appropriate to examine the possible role of free radicals in brain discrete regions and the ＂antioxidants＂ mediated response of S. dulcis. Animals were subjected to noise stress for 15 days （100 dB/4 hours/day） and estimation of endogenous free radical and antioxidant activity were carried out on brain discrete regions （the cerebral cortex, cerebellum, brainstem, striatum, hippocampus and hypothalamus）. The result showed that exposure to noise could alleviate endogenous free radical generation and altered anfioxidant status in brain discrete regions when compared to that of the control groups. This alleviated free radical generation （H202 and NO） is well supported by an upregulated protein expression on immunohistochemistry of both iNOS and nNOS in the cerebral cortex on exposure to noise stress. These findings suggest that increased free radical generation and altered anti-oxidative status can cause redox imbalance in the brain discrete regions~ However, free radical scavenging activity of the plant was evident as the noise exposed group treated with S. dulcis[200 mg/（kg · b. w）] displayed a therapeutic effect by decreasing the free radical level and regulate the anti-oxidative status to that of control animals. Hence, it can be concluded that the efficacy of S. dulcis could be attributed to its free radical scavenging activity and anti-oxidative property.