Identification of Sperm Subpopulations in Water Buffalo Ejaculates： Changes in Cryopreservation Stages and Bull Variation

This study aimed to identify and characterize the sperm subpopulations existing in water buffalo semen using a computer assisted sperm analyzer （CASA）, as well as assess the effects of cryopreservation on the sperm subpopulation structure and evaluate bull variability. The semen of eight Bulgarian M...

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Bibliographic Details
Published in:农业科学与技术：A 2017, Vol.7 (3), p.184-192
Main Author: Excel Rio Santos Maylem Maria Elizabeth Dela Cruz Leoveras Emma Villanueva Venturina Eufrocina Dela Pena Atabay Edwin Curading Atabay
Format: Article
Language:Chinese
Subjects:
公牛
冷冻保存
子群
射精
水牛
精子运动
计算机辅助
识别
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Summary:This study aimed to identify and characterize the sperm subpopulations existing in water buffalo semen using a computer assisted sperm analyzer （CASA）, as well as assess the effects of cryopreservation on the sperm subpopulation structure and evaluate bull variability. The semen of eight Bulgarian Murrah bulls was collected by four times in an interval of one week each. The semen was cryopreserved following a standard protocol and sperm kinematics was assessed. Clustering methods were applied to individual sperms, forming two significantly different （P 〈 0.05） subpopulations （P1 and P2）. Subpopulation P1 represents those spermatozoa that moved most rapidly and progressively （46.29%）, and subpopulation P2 includes spermatozoa with relatively low velocity or poorly motile but with high progressiveness （53.41%）. There was a decline on the population of P1 sperms from fresh （52.52%）, pre-freeze （45.73%） to post-thaw （35.17%） stages and significant difference on the sperm kinematics between P1 and P2. A significan
ISSN:2161-6256