Ran binding protein 9（RanBPM） binds IFN-λR1 in the IFN-λsignaling pathway

• Published in: 中国科学：生命科学英文版 2017, Vol.60 (9), p.1030-1039
• Main Author: Junwen Zhang XiaojieCong Jiajie Zhaoqiao Xia Yang Meng Li Hong Chen Ruifang Mi Guishan Jin Fusheng Liu Bing-Ren Huang
• Format: Article
• Language: English
• Subjects: IFN-γ; Ran; γ干扰素; 信号通路; 免疫共沉淀; 异源二聚体; 相互作用; 结合蛋白
• ISSN: 1674-7305; 1869-1889

Like the type I interferons（IFNs）,the recently discovered cytokine IFN-λ displays antiviral,antiproliferative,and proapoptotic activities,mediated by a heterodimeric IFN-λ receptor complex composed of a unique IFN-λR1 chain and the IL-10R2 chain.However,the molecular mechanism of the IFN-λ-regulated pathway remains unclear.In this study,we newly identified RAN-binding protein M（RanBPM） as a binding partner of IFN-λR1.The interaction between RanBPM and IFN-λRl was identified with a glutathione S-transferase pull-down assay and coimmunoprecipitation experiments.IFN-λ1 stimulates this interaction and affects the cellular distribution of RanBPM.However,the interaction between RanBPM and IFN-λR1 does not correlate with their conserved TRAF6-binding sites.Furthermore,we also found that RanBPM is a scaffolding protein with a modulatory function that regulates the activities of IFN-stimulated response elements.Therefore,RanBPM plays a novel role in the IFN-λ-regulated signaling pathway.