DMSO-离子液体共溶剂强化咖啡酸苯乙酯的酶促合成（英文）

• Published in: 中国化学工程学报：英文版 2014 (Z1), p.1314-1321
• Main Author: 顾双双 王俊 魏贤彬 崔红生 吴向阳 吴福安
• Format: Article
• Language: English
• Subjects: acid; Biocatalysis; Caffeic; Co-solvent; ester; Ionic; Kinetics; liquid; phenethyl
• ISSN: 1004-9541; 2210-321X

Caffeic acid phenethyl ester(CAPE) is a natural and rare ingredient with several biological activities, but its industrial production using lipase-catalyzed esterification of caffeic acid(CA) and 2-phenylethanol(PE) in ionic liquids(ILs) is hindered by low substrate concentrations and long reaction time. To set up a high-efficiency bioprocess for production of CAPE, a novel dimethyl sulfoxide(DMSO)–IL co-solvent system was established in this study.The 2%(by volume) DMSO–[Bmim][Tf2N] system was found to be the best medium with higher substrate solubility and conversion of CA. Under the optimum conditions, the substrate concentration of CA was raised 8-fold,the reaction time was reduced by half, and the conversion reached 96.23%. The kinetics follows a ping-pong bi-bi mechanism with inhibition by PE, with kinetic parameters as follows: Vmax= 0.89 mmol · min-1· g-1, Km,CA=42.9 mmol · L-1, Km,PE= 165.7 mmol · L-1, and Ki,PE= 146.2 mmol · L-1. The results suggest that the DMSO cosolvent effect has great potential to enhance the enzymatic synthesis efficiency of CAPE in ILs.