The immunophilin Zonda controls regulated exocytosis in endocrine and exocrine tissues

Exocytosis is a fundamental process in physiology, that ensures communication between cells, organs and even organisms. Hormones, neuropeptides and antibodies, among other cargoes are packed in exocytic vesicles that need to reach and fuse with the plasma membrane to release their content to the ext...

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Bibliographic Details
Published in:Traffic (Copenhagen, Denmark) Denmark), 2021-04, Vol.22 (4), p.111-122
Main Authors: de la Riva‐Carrasco, Rocío, Perez‐Pandolfo, Sebastián, Suárez Freire, Sofía, Romero, Nuria M., Bhujabal, Zambarlal, Johansen, Terje, Wappner, Pablo, Melani, Mariana
Format: Article
Language:English
Subjects:
Autophagy
Cell interactions
Cell Membrane
Drosophila
Exocytosis
Genetic screening
Immunophilin
Immunophilins
Insulin
Neuropeptides
Phagocytosis
RalA
Ring gland
Salivary gland
Secretory granule
Secretory Vesicles
Zonda
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Summary:Exocytosis is a fundamental process in physiology, that ensures communication between cells, organs and even organisms. Hormones, neuropeptides and antibodies, among other cargoes are packed in exocytic vesicles that need to reach and fuse with the plasma membrane to release their content to the extracellular milieu. Hundreds of proteins participate in this process and several others in its regulation. We report here a novel component of the exocytic machinery, the Drosophila transmembrane immunophilin Zonda (Zda), previously found to participate in autophagy. Zda is highly expressed in secretory tissues, and regulates exocytosis in at least three of them: the ring gland, insulin‐producing cells and the salivary gland. Using the salivary gland as a model system, we found that Zda is required at final steps of the exocytic process for fusion of secretory granules to the plasma membrane. In a genetic screen we identified the small GTPase RalA as a crucial regulator of secretory granule exocytosis that is required, similarly to Zda, for fusion between the secretory granule and the plasma membrane. Synopsis The immunophilin Zonda acts at the final stages of exocytosis by regulating fusion of the secretory granule to the apical plasma membrane.
ISSN:1398-9219
1600-0854
1600-0854
DOI:10.1111/tra.12777