Dried blood spot analysis with liquid chromatography and mass spectrometry: Trends in clinical chemistry

Dried blood spot samples are simple to prepare and transport, enabling safe and accessible diagnostics, both locally and globally. We review dried blood spot samples for clinical analysis, focusing on liquid chromatography‐mass spectrometry as a versatile measurement tool for these samples. Dried bl...

Full description

Saved in:
Bibliographic Details
Published in:Journal of separation science 2023-08, Vol.46 (15), p.e2300210-n/a
Main Authors: Skogvold, Hanne Bendiksen, Rootwelt, Helge, Reubsaet, Léon, Elgstøen, Katja Benedikte Prestø, Wilson, Steven Ray
Format: Article
Language:English
Subjects:
Analytical chemistry
Antibodies
Blood
Chemistry, Clinical
Chromatography
Chromatography, Liquid - methods
Column chromatography
dried blood spot
Dried Blood Spot Testing - methods
Humans
Infant, Newborn
Ions
Liquid chromatography
Mass spectrometry
Medical screening
omics
Proteomics
Scientific imaging
Specimen Handling
Tandem Mass Spectrometry - methods
xenobiotics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Dried blood spot samples are simple to prepare and transport, enabling safe and accessible diagnostics, both locally and globally. We review dried blood spot samples for clinical analysis, focusing on liquid chromatography‐mass spectrometry as a versatile measurement tool for these samples. Dried blood spot samples can provide information for, for example, metabolomics, xenobiotic analysis, and proteomics. Targeted analyses of small molecules are the main application of dried blood spot samples and liquid chromatography‐mass spectrometry, but emerging applications include untargeted metabolomics and proteomics. Applications are highly varied, including analyses related to newborn screening, diagnostics and monitoring of disease progression and treatment effects of virtually any disease, as well as studies into the physiology and effects of diet, exercise, xenobiotics, and doping. A range of dried blood spot products and methods are available, and applied liquid chromatography‐mass spectrometry instrumentation is varied with regard to liquid chromatography column formats and selectivity. In addition, novel approaches such as on‐paper sample preparation (e.g., selective trapping of analytes with paper‐immobilized antibodies) are described. We focus on research papers published in the last 5 years.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.202300210