The edible mushroom Albatrellus ovinus contains a α-l-fuco-α-d-galactan, α-d-glucan, a branched (1 → 6)-β-d-glucan and a branched (1 → 3)-β-d-glucan

• Published in: Carbohydrate research 2019-01, Vol.471, p.28-38
• Main Authors: Samuelsen, Anne Berit C., Rise, Frode, Wilkins, Alistair L., Teveleva, Lioubov, Nyman, Anna Armika Tussilago, Aachmann, Finn L.
• Format: Article
• Language: English
• Subjects: Albatrellus ovinus; Fucogalactan; α-glucan; β-glucan
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/j.carres.2018.10.012

Albatrellus ovinus, the sheep polypore, is a large, dense mushroom being rich in cell wall material. Polysaccharides were isolated by sequential extraction, enzymatic treatment and analyzed with respect to monosaccharide composition, glycosidic linkages by methylation and GC-MS as well as NMR spectroscopy. A fucogalactan composed of an (1 → 6)-α-d-galactan backbone with single α-l-Fucp residues attached at O-2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography. Both the hot water extract and the hot alkali extract contained an (1 → 4)-α-d-glucan whereas β-d-glucans were mainly present in the latter. Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6. In addition there were identified short (1 → 2)-linked β-d-xylan and (1 → 3)-α-d-mannan chains. [Display omitted] •The sheep polypore (Albatrellus ovinus) is considered an edible mushroom.•A.ovinus contains at least four different extractable cell wall polysaccharides.•The water soluble polysaccharides includes a fucogalactan and a glycogen-like α-d-glucan.•A.ovinus contains a branched (1. → 6)-β-d-glucan and (1 → 3)-linked β-d-glucan in addition to chains of α-d-mannan and β-d-xylan