NETs analysed by novel calprotectin‐based assays in blood donors and patients with multiple myeloma or rheumatoid arthritis: A pilot study

Two novel enzyme‐linked immunosorbent assays (ELISAs), designed to detect complexes containing DNA, leucocyte calprotectin and S100A12 proteins, were generated for improved specificity and rapid measurement of neutrophil extracellular traps (NETs). The assays were applied on plasma and serum samples...

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Bibliographic Details
Published in:Scandinavian journal of immunology 2020-05, Vol.91 (5), p.e12870-n/a
Main Authors: Fagerhol, Magne Kristoffer, Johnson, Egil, Tangen, Jon‐Magnus, Hollan, Ivana, Mirlashari, Mohammad Reza, Nissen‐Meyer, Lise Sofie Haug, Hetland, Geir
Format: Article
Language:English
Subjects:
Adult
Aged
Arthritis, Rheumatoid - etiology
Arthritis, Rheumatoid - metabolism
Arthritis, Rheumatoid - pathology
Blood Donors
calprotectin
Case-Control Studies
Enzyme-Linked Immunosorbent Assay
Extracellular Traps - immunology
Extracellular Traps - metabolism
Female
Humans
Inflammation
Leukocyte L1 Antigen Complex - metabolism
Male
Middle Aged
Multiple myeloma
Multiple Myeloma - etiology
Multiple Myeloma - metabolism
Multiple Myeloma - pathology
NETs
Neutrophils - immunology
Neutrophils - metabolism
Neutrophils - pathology
Pilot Projects
Rheumatoid arthritis
Young Adult
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Summary:Two novel enzyme‐linked immunosorbent assays (ELISAs), designed to detect complexes containing DNA, leucocyte calprotectin and S100A12 proteins, were generated for improved specificity and rapid measurement of neutrophil extracellular traps (NETs). The assays were applied on plasma and serum samples from blood donors for establishment of reference values, and from patients with multiple myeloma (MM) or rheumatoid arthritis (RA) in order to examine putatively increased values in the two different inflammatory conditions. Although NETs were hardly detectable in healthy individuals, NET levels were as expected highly and statistically significantly increased in RA patients. The detection of statistically significantly increased NET levels in MM is a novel finding.
ISSN:0300-9475
1365-3083
DOI:10.1111/sji.12870