Ancient DNA sequence quality is independent of fish bone weight

The field of ancient DNA (aDNA) typically uses between 50 and 200 mg of minimum input weight of bone material for the extraction of DNA from archaeological remains. While laboratory and analysis techniques have focused on improved efficiency of extracting useable sequence data from older and poorer...

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Main Authors: Atmore, Lane Margaret, Ferrari, Giada, Martínez-Garciá, Lourdes, van der Jagt, Inge, Blevis, Rachel, Granado, José, Häberle, Simone, Dierickx, Katrien, Quinlan, Liz M, Lõugas, Lembi, Makowiecki, Daniel, Hufthammer, Anne Karin, Barrett, James, Star, Bastiaan
Format: Article
Language:English
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Summary:The field of ancient DNA (aDNA) typically uses between 50 and 200 mg of minimum input weight of bone material for the extraction of DNA from archaeological remains. While laboratory and analysis techniques have focused on improved efficiency of extracting useable sequence data from older and poorer quality remains, bone material input requirements have rarely been critically evaluated. Here, we present the aDNA analysis of 121 size-constrained Atlantic herring remains – weighing between