Growth Associated Protein-43 mRNA Expression in Nucleus Ambiguus Motoneurons after Recurrent Laryngeal Nerve Injury in the Rat

Objective-To investigate the time-dependent differences in growth associated protein-43 (GAP-43) mRNA expression in the nucleus ambiguus (NA) motoneurons after recurrent laryngeal nerve (RLN) transection in the rat. Material and Methods-Animals were sacrificed on Days 1, 7, 14 and 21 after axotomy....

Full description

Saved in:
Bibliographic Details
Published in:Acta oto-laryngologica 2003-02, Vol.123 (2), p.292-296, Article 292
Main Authors: Uno, Toshiyuki, Shogaki, Kazuhiro, Bamba, Hitoshi, Koike, Shinobu, Naruse, Yoshihisa, Ijima, Norio, Tanaka, Masaki, Hisa, Yasuo
Format: Article
Language:English
Subjects:
Animals
Axotomy
Base Sequence
Biological and medical sciences
Disease Models, Animal
Ent, stomatology, face, injuries. Foreign bodies. Diseases due to physical agents: otorhinolaryngology
GAP-43 Protein - genetics
Gene Expression Regulation
In Situ Hybridization
Male
Medical sciences
Medulla Oblongata - metabolism
Molecular Sequence Data
Motor Neurons - metabolism
Nerve Regeneration - genetics
nitric oxide synthase
Nitric Oxide Synthase - metabolism
Peripheral Nerves
Polymerase Chain Reaction
Random Allocation
Rats
Rats, Sprague-Dawley
Recurrent Laryngeal Nerve Injuries
Reference Values
regeneration
RNA, Messenger - analysis
Sensitivity and Specificity
Traumas. Diseases due to physical agents
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective-To investigate the time-dependent differences in growth associated protein-43 (GAP-43) mRNA expression in the nucleus ambiguus (NA) motoneurons after recurrent laryngeal nerve (RLN) transection in the rat. Material and Methods-Animals were sacrificed on Days 1, 7, 14 and 21 after axotomy. The sections were then processed for in situ hybridization of GAP-43 mRNA. Results-GAP-43 mRNA transiently increased after axotomy, reaching a peak on postoperative Day 7, and then decreased gradually at 14 and 21 days after axotomy. The peak period of GAP-43 expression in the NA is different from those in the facial and hypoglossal nuclei after axotomy. Conclusion-We have already reported that neuronal nitric oxide synthase (nNOS) is induced in the NA motoneurons after axotomy and reaches a peak on postoperative Day 14. This study shows that the expressions of GAP-43 and nNOS are chronologically different.
ISSN:0001-6489
1651-2251
DOI:10.1080/00016480310001150