Interaction properties of human TRAMP‐like proteins and their role in pre‐rRNA 5′ETS turnover

In yeast, the Trf4/5‐Air1/2‐Mtr4 polyadenylation (TRAMP) complex acts as a cofactor for the nuclear exosome to promote degradation of various RNAs. However, the corresponding machinery in mammals is less characterized. We analyzed the interactions of the human TRAMP‐like proteins, PAPD5, ZCCHC7, and...

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Bibliographic Details
Published in:FEBS letters 2016-09, Vol.590 (17), p.2963-2972
Main Authors: Sudo, Haruka, Nozaki, Aya, Uno, Hideaki, Ishida, Yo‐ichi, Nagahama, Masami
Format: Article
Language:English
Subjects:
exosome
PAP‐associated domain‐containing protein 5
pre‐rRNA
RRP6
Trf4/5‐Air1/2‐Mtr4 polyadenylation
zinc finger CCHC domain‐containing protein 7
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Summary:In yeast, the Trf4/5‐Air1/2‐Mtr4 polyadenylation (TRAMP) complex acts as a cofactor for the nuclear exosome to promote degradation of various RNAs. However, the corresponding machinery in mammals is less characterized. We analyzed the interactions of the human TRAMP‐like proteins, PAPD5, ZCCHC7, and MTR4, with the nuclear exosome. PAPD5 and ZCCHC7 exhibited mutual interactions in presence of the exosome catalytic subunit RRP6, whereas MTR4 was dispensable for their assembly. Furthermore, the human TRAMP‐like proteins were involved in the RRP6‐catalyzed turnover of pre‐rRNA 5′ETS fragments. These results suggest the significant role for RRP6 in the assembly of TRAMP‐like proteins during nucleolar RNA surveillance.
ISSN:0014-5793
1873-3468
DOI:10.1002/1873-3468.12314