Advanced Hierarchical Computational Modeling‐Based Rational Development of Platinum (II) Nanocomplex to Improve Lung Cancer Therapy

• Published in: Advanced functional materials 2024-09
• Main Authors: Huang, Haozhe, Ji, Beihong, Huang, Yixian, Li, Shichen, Luo, Zhangyi, Chen, Shangyu, Li, Sihan, Chen, Yuang, Bain, Daniel J., Sun, Jingjing, Yang, Da, Burns, Timothy F., Wang, Junmei, Li, Song
• Format: Article
• Language: English
• ISSN: 1616-301X; 1616-3028
• DOI: 10.1002/adfm.202411334

Cancer stem cells (CSCs) are known to be one of the determining factors that contribute to therapeutic resistance. However, much remains to be understood about the reprogramming network leading to the generation of CSCs driven by chemotherapy. In this study, guided by bioinformatics study, deeper insight is uncovered and provided into the CSC enrichment mechanism driven by cisplatin (CDDP) treatment. It is discovered that CDDP can repopulate the level of CSC by activating AKT1 oncogenic pathway that is further enhanced by COX‐2 inflammatory signaling. Simultaneously blocking these two pathways can synergistically restrain the number of CSCs. Under the guidance of advanced hierarchical computational modeling, including molecular docking, molecular dynamics (MD) simulation and binding free energy analysis, MK‐2206 is selected as the AKT1 inhibitor to achieve optimal codelivery of CDDP, MK‐2206 and 5‐ASA (COX‐2 inhibitor) through 5‐ASA‐derivatized dual functional immunostimulatory nanocarrier (PASA). This triple combination (PASA/CDDP/MK‐2206) significantly reduces tumor burden in both orthotopic and metastatic lung cancer models. Mechanistic studies show that this improved therapeutic activity is due to elimination of CSCs and reversal of the immunosuppressive tumor microenvironment. This study suggests that PASA/CDDP/MK‐2206 may represent a simple and effective lung cancer therapy via reversing CSCs‐associated chemoresistance.