Mifepristone Modulates the Polarization of Th1/Th2 by Inducing the Mature of Monocyte‐Derived Dendritic Cells

• Published in: Advanced therapeutics 2024-02, Vol.7 (2), p.n/a
• Main Authors: Li, Yinghua, Li, Li, Li, Songyi, He, Chaying
• Format: Article
• Language: English
• Subjects: dendritic cells; mifepristone; Th1; Th2
• ISSN: 2366-3987; 2366-3987
• DOI: 10.1002/adtp.202300245

As an anti‐implantation contraception medicine, mifepristone can alter the development of the endometrium. In contrast, little research is performed to decipher the effect of mifepristone on Th1/Th2 immune homeostasis in the endometrium. CD14+ monocytes derived from healthy donors are cultured with interleukin (IL)‐4 and granulocyte macrophage‐colony stimulating factor to induce monocyte‐derived dendritic cells (DCs), which are further incubated with mifepristone. Mifepristone incubation could induce up‐regulated CD83 and major histocompatibility complex cell surface receptor staining on monocyte‐derived DCs. Functionally, mifepristone incubation could enhance the allostimulatory capacity of DCs to promote the proliferation of allogeneic T cells with up‐regulated Th1 cell proportion and down‐regulated Th2 cell proportion. On the other side, increased Th1 cytokines release (interferon‐γ, tumor necrosis factor α), inhibited Th2 cytokines release (IL‐10, IL‐4), promoted T‐Box transcription factor 21 transcription, and inhibited GATA binding protein 3 transcription are also identified. Mifepristone‐stimulated DCs could alter the Th1/Th2 transcription balance to favor Th1 skewing inflammatory environment. Mifepristone induces the maturation of monocyte‐derived dendritic cells, and enhances the allostimulatory capacity of the dendritic cells. Mifepristone‐stimulated dendritic cells promote Th1 cytokine release but inhibit Th2 cytokine release, as well as regulate the Th1/Th2 transcription balance. Mifepristone‐stimulated dendritic cells could alter the Th1/Th2 transcription balance to favor Th1 skewing inflammatory environment.