Immobilization of glucose oxidase in chitosan gel beads

Glucose oxidase was conjugated to the biopolymer chitosan using carbodiimide conjugation. Gel beads were formed from the enzyme–biopolymer complex, and the activity of the immobilized enzyme was compared to the method of encapsulating the enzyme in chitosan. The activity of the immobilized enzyme wa...

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Bibliographic Details
Published in:Journal of applied polymer science 2004-01, Vol.91 (2), p.861-866
Main Authors: Ghanem, Amyl, Ghaly, Ahmed
Format: Article
Language:English
Subjects:
Applied sciences
biopolymers
chitosan
enzymes
Exact sciences and technology
gels
glucose oxidase
Physicochemistry of polymers
Polymer industry, paints, wood
Technology of polymers
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Summary:Glucose oxidase was conjugated to the biopolymer chitosan using carbodiimide conjugation. Gel beads were formed from the enzyme–biopolymer complex, and the activity of the immobilized enzyme was compared to the method of encapsulating the enzyme in chitosan. The activity of the immobilized enzyme was found to increase with increasing carbodiimide concentration and time of incubation, to a maximum. Enzyme activity decreased with time as the beads were air‐dried. The pH optima of the immobilized enzyme was shifted to a more acidic value compared to that of the free enzyme. The use of carbodiimide chemistry to conjugate glucose oxidase to a chitosan gel was demonstrated. This technique holds promise for the development of immobilized enzymes for applications in medicine, biosensors, and bioprocessing. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 91: 861–866, 2004
ISSN:0021-8995
1097-4628
DOI:10.1002/app.13221