Modulation of steroidogenesis by selenium in a novel adrenal cell line developed using targeted tumorigenesis

Glutathione peroxidase (GPx‐1) is a selenoenzyme that metabolizes H2O2, a source of potentially toxic free radicals. Steroidogenesis is markedly inhibited by H2O2 in vitro. Objective: to study the effects of selenium deficiency on GPx activity and adrenal steroidogenesis in a novel adrenal cell line...

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Bibliographic Details
Published in:BioFactors (Oxford) 2001, Vol.14 (1-4), p.229-238
Main Authors: Chanoine, Jean-Pierre, Compagnone, Nathalie A., Wong, Alfred C. K., Mellon, Synthia H.
Format: Article
Language:English
Subjects:
8-Bromo Cyclic Adenosine Monophosphate - pharmacology
adrenal cells
Adrenal Cortex - cytology
Adrenal Cortex - drug effects
Adrenal Cortex - physiology
Aldosterone - biosynthesis
Animals
Cell Line
Cell Transformation, Neoplastic
Cholesterol Side-Chain Cleavage Enzyme - genetics
Cholesterol Side-Chain Cleavage Enzyme - metabolism
Corticosterone - biosynthesis
Glutathione Peroxidase - metabolism
glutathione peroxidase activity
H_2O_2
Humans
Hydrogen Peroxide - metabolism
Mice
Mice, Transgenic
Phosphoproteins - genetics
Phosphoproteins - metabolism
Progesterone - biosynthesis
Promoter Regions, Genetic
Reverse Transcriptase Polymerase Chain Reaction
selenium
Selenium - deficiency
Selenium - pharmacology
steroidogenesis
Transcription, Genetic
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Summary:Glutathione peroxidase (GPx‐1) is a selenoenzyme that metabolizes H2O2, a source of potentially toxic free radicals. Steroidogenesis is markedly inhibited by H2O2 in vitro. Objective: to study the effects of selenium deficiency on GPx activity and adrenal steroidogenesis in a novel adrenal cell line developed using targeted tumorigenesis. Methods: AN4Rppc7 cells were grown for 7 days in serum‐free medium. 8‐Br‐cAMP‐stimulated concentrations of steroid hormones were measured by RIA. StAR (Steroid Acute Reactive Protein) mRNA was measured by Northern blot. Results: selenium deficiency caused a 99% There was a 51%, progesterone, corticosterone and aldosterone production, respectivelyp
ISSN:0951-6433
1872-8081
DOI:10.1002/biof.5520140129