The simultaneous biosynthesis and uptake of amino acids by Lactococcus lactis studied by 13 C‐labeling experiments

Uniformly 13 C labeled glucose was fed to a lactic acid bacterium growing on a defined medium supplemented with all proteinogenic amino acids except glutamate. Aspartate stemming from the protein pool and from the extracellular medium was enriched with 13 C disclosing a substantial de novo biosynthe...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2002-04, Vol.78 (1), p.11-16
Main Authors: Jensen, Niels Bang Siemsen, Christensen, Bjarke, Nielsen, Jens, Villadsen, John
Format: Article
Language:English
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Summary:Uniformly 13 C labeled glucose was fed to a lactic acid bacterium growing on a defined medium supplemented with all proteinogenic amino acids except glutamate. Aspartate stemming from the protein pool and from the extracellular medium was enriched with 13 C disclosing a substantial de novo biosynthesis of this amino acid simultaneous to its uptake from the growth medium and a rapid exchange flux of aspartate over the cellular membrane. Phenylalanine, alanine, and threonine were also synthesized de novo in spite of their presence in the growth medium. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 11–16, 2002; DOI 10.1002/bit.10211
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.10211