A ventilated germ-free cabinet for the microbiological laboratory

The design of a cabinet for the aseptic manipulation of material is described. The interior surfaces of the cabinet are sterilized by swabbing with a germicide. Germ‐free conditions are maintained during operation by means of a positive pressure of sterile air. A fan and filter unit attached to the...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1963-03, Vol.5 (1), p.53-58
Main Authors: Harris-Smith, R., Pirt, S. J., Firman, J. E.
Format: Article
Language:English
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Summary:The design of a cabinet for the aseptic manipulation of material is described. The interior surfaces of the cabinet are sterilized by swabbing with a germicide. Germ‐free conditions are maintained during operation by means of a positive pressure of sterile air. A fan and filter unit attached to the cabinet is used to blow sterile air into the cabinet in sufficient quantity so as to flow out of the hand ports at a velocity of 50 ft./min. In the design, careful consideration was given to the ease of manipulations in the cabinet.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260050109