Interfacial phenomena governing adhesion of chlorella to glass surfaces

• Published in: Biotechnology and bioengineering 1967-10, Vol.9 (4), p.545-558
• Main Authors: Nordin, John S., Tsuchiya, H. M., Fredrickson, A. G.
• Format: Article
• Language: English
• ISSN: 0006-3592; 1097-0290
• DOI: 10.1002/bit.260090409

Interfacial phenomena are directly involved in the adhesion of a strain of Chlorella, a unicellular alga, to glass surfaces in simple ionic solutions. The principal mechanisms governing the adhesion appear to be electrostatic interaction between electrical double layers and various specific surface interactions resulting from surface heterogeneity and ion adsorption. Under most conditions the algal cells and glass surfaces have negative zeta potentials, and adhesion to glass will not occur; but if, for example, FeCl3 is added to an algal–glass system immersed in 0.05M NaCl, the algal and glass surfaces will possess very different zeta potentials, and adhesion will be strongest under those conditions which produce the greatest, difference in zeta poentials. Prior pretreatment and usage of glass apparatus greatly affect the glass zeta potentials and the adhesion of algal cells to glass. An apparatus for measuring a relative set of numbers representing the force of adhesion of algal cells is described.