unique biopolymer from Rhinocladiella mansonii NRRL Y 6272: production in 20 liter fermentors

The black yeast‐like fungus Rhinocladiella mansonii NRRL Y‐6272 elaborates an extracellular polysaccharide composed of N‐acetyl D‐glucosamine and N‐acetyl D‐glucosaminuronic acid residues in a 2:1 molar ratio. Production of this polymer was studied in 300 ml flasks and 20 liter fermentors. On the ba...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1976-12, Vol.18 (12), p.1669-1677
Main Authors: Burton, K.A, Cadmus, M.C, Lagoda, A.A, Sandford, P.A, Watson, P.R
Format: Article
Language:English
Subjects:
plant biochemistry
plant physiology
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Summary:The black yeast‐like fungus Rhinocladiella mansonii NRRL Y‐6272 elaborates an extracellular polysaccharide composed of N‐acetyl D‐glucosamine and N‐acetyl D‐glucosaminuronic acid residues in a 2:1 molar ratio. Production of this polymer was studied in 300 ml flasks and 20 liter fermentors. On the basis of glucose utilized, 13% conversion to polymer was obtained in four days at 25°C in medium containing yeast autolysate paste, K2HPO4, MgSO4, ZnSO4, L‐asparagine or urea, and D‐glucose. Nitrogen sources, aeration, agitation, temperature, and salts affected yields. The polymer in diluted fermentation broths was precipitated with ethanol in the presence of an electrolyte and collected by centrifugation. The viscosities of aqueous resolutions containing 1 and 1.5% of the polymer were 9400 cP and 21,500 cP, respectively.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260181203