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Pulse response in an immobilized-enzyme column: elution profiles in reversible and consecutive reactions [Concentrations of substances in food]

A pulse of substrate solution was applied to an immobilized‐enzyme column, in which the substrate was then converted by reversible or consecutive reactions. Immobilized glucose isomerase was used for the reversible reaction, and immobilized invertase and glucose oxidase for the consecutive reactions...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1981-09, Vol.23 (9), p.1961-1976
Main Authors: Adachi, S, Hashimoto, K, Miyai, K, Kurome, H, Kamikubo, T
Format: Article
Language:English
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Summary:A pulse of substrate solution was applied to an immobilized‐enzyme column, in which the substrate was then converted by reversible or consecutive reactions. Immobilized glucose isomerase was used for the reversible reaction, and immobilized invertase and glucose oxidase for the consecutive reactions. The elution profiles of substrate and product were determined experimentally. These profiles were in good agreement with the ones predicted theoretically. The effect of some parameters on the elution profiles for reversible and consecutive reactions is discussed.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260230903