Optimization of kinetic parameters of enzymes

Enzymes are a product of evolution, having achieved a high specificity and catalytic performance by means of natural selection. Because catalytic mechanisms are very complex, a detailed and rigorous analysis of their optimization functions, as it has been developed in our previous work, is rather di...

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Bibliographic Details
Published in:Biochemistry and molecular biology education 2002-05, Vol.30 (3), p.184-188
Main Authors: Heinrich, Reinhart, Meléndez‐Hevia, Enrique, Cabezas, Héctor
Format: Article
Language:English
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Summary:Enzymes are a product of evolution, having achieved a high specificity and catalytic performance by means of natural selection. Because catalytic mechanisms are very complex, a detailed and rigorous analysis of their optimization functions, as it has been developed in our previous work, is rather difficult for teaching purposes. In this paper we present a summarized and simplified view of our results by means of analyzing the optimization of kinetic parameters of a two‐step enzyme mechanism under a set of several constraints. This analysis is presented in a way that makes it useful for teaching, as students can understand it easily, make the calculations, and even develop it by exploring new environmental conditions. The analysis of the optimization processes is presented through two calculation tools, analysis and computer programming.
ISSN:1470-8175
1539-3429
DOI:10.1002/bmb.2002.494030030065