Development and validation of automated SPE-HPLC-MS/MS methods for the quantification of asenapine, a new antipsychotic agent, and its two major metabolites in human urine

ABSTRACT To support the evaluation of the pharmacokinetic parameters of asenapine (ASE) in urine, we developed and validated online solid‐phase extraction high‐performance liquid chromatography methods with tandem mass spectrometry detection (SPE‐LC‐MS/MS) for the quantification of ASE and two of it...

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Bibliographic Details
Published in:Biomedical chromatography 2012-12, Vol.26 (12), p.1461-1463
Main Authors: de Boer, Theo, Meulman, Erik, Meijering, Henri, Wieling, Jaap, Dogterom, Peter, Lass, Holger
Format: Article
Language:English
Subjects:
Antipsychotic Agents - pharmacokinetics
Antipsychotic Agents - urine
asenapine
automated
Chromatography, High Pressure Liquid - methods
Double-Blind Method
Heterocyclic Compounds, 4 or More Rings - pharmacokinetics
Heterocyclic Compounds, 4 or More Rings - urine
Humans
liquid chromatography-tandem mass spectrometry
quantification
Reproducibility of Results
Sensitivity and Specificity
Solid Phase Extraction - methods
solid-phase extraction
Tandem Mass Spectrometry - methods
urine
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Summary:ABSTRACT To support the evaluation of the pharmacokinetic parameters of asenapine (ASE) in urine, we developed and validated online solid‐phase extraction high‐performance liquid chromatography methods with tandem mass spectrometry detection (SPE‐LC‐MS/MS) for the quantification of ASE and two of its major metabolites, N‐desmethylasenapine (DMA) and asenapine‐N+‐glucuronide (ASG). The linearity in human urine was found acceptable for quantification in a concentration range of 0.500–100 ng/mL for ASE and DMA and 10.0–3000 ng/mL for ASG, respectively. Copyright © 2012 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.2722