A fast, cost‐saving and sensitive method for determination of cefuroxime in plasma by HPLC with ultraviolet detection

Cefuroxime (CFX) is a broad‐spectrum second‐generation cephalosporin and one of the best choices for antibiotic prophylaxis. However, when used in critically ill patients, it may present changes in its pharmacokinetic properties. Therefore, therapeutic drug monitoring of CFX is necessary for effecti...

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Bibliographic Details
Published in:Biomedical chromatography 2021-10, Vol.35 (10), p.n/a
Main Authors: Hernandis, Verónica, Escudero, Elisa, Pareja, Ana, Marín, Pedro
Format: Article
Language:English
Subjects:
cefuroxime
cephalosporins
HPLC
pharmacokinetics
plasma
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Summary:Cefuroxime (CFX) is a broad‐spectrum second‐generation cephalosporin and one of the best choices for antibiotic prophylaxis. However, when used in critically ill patients, it may present changes in its pharmacokinetic properties. Therefore, therapeutic drug monitoring of CFX is necessary for effective dosing strategies. A simple, rapid and sensitive liquid chromatographic method with UV detection was developed and validated for the quantification of CFX in plasma. The method involved a single‐step precipitation of proteins with methanol and trifluoroacetic acid. Cefuroxime was analyzed on a Brisa LC2C18 column in isocratic mode consisting of 0.1% trifluoroacetic acid in water and acetonitrile (75:25) with UV detection at a wavelength of 280 nm. The retention times of CFX and cephazolin (internal standard) were 9.8 and 7.4 min, respectively. The calibration curve was linear over a concentration range of 0.25–50 μg/ml. The limits of detection and quantification were 0.1 μg/ml and 0.25 μg/ml, respectively. The accuracy and precision were always
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.5188