Elucidation of the Interactions of an Anticancer Ruthenium Complex in Clinical Trials with Biomolecules Utilizing Capillary Electrophoresis Hyphenated to Inductively Coupled Plasma-Mass Spectrometry. Short Communication

The application of capillary electrophoresis (CE) combined with highly sensitive inductively‐coupled‐plasma mass spectrometric (ICP‐MS) detection allows the interactions of metal complexes with biomolecules to be characterized. This technique has been used to provide new insights into the mode of ac...

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Bibliographic Details
Published in:Chemistry & biodiversity 2008-08, Vol.5 (8), p.1609-1614
Main Authors: Groessl, Michael, Hartinger, Christian G., Połeć-Pawlak, Kasia, Jarosz, Maciej, Dyson, Paul J., Keppler, Bernhard K.
Format: Article
Language:English
Subjects:
Anticancer research
Antineoplastic Agents - blood
Antineoplastic Agents - chemistry
Antineoplastic Agents - pharmacology
Binding Sites
Capillary electrophoresis (CE)
Clinical Trials as Topic
Deoxyguanine Nucleotides - chemistry
DNA
DNA - chemistry
Electrophoresis, Capillary - methods
Humans
Indazoles - blood
Indazoles - chemistry
Indazoles - pharmacology
KP1019
Mass spectrometry
Mass Spectrometry - methods
Molecular Conformation
Organometallic Compounds
Ruthenium complexes
Ruthenium Compounds - blood
Ruthenium Compounds - chemistry
Ruthenium Compounds - pharmacology
Serum Albumin - chemistry
Spectrophotometry, Ultraviolet
Structure-Activity Relationship
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Summary:The application of capillary electrophoresis (CE) combined with highly sensitive inductively‐coupled‐plasma mass spectrometric (ICP‐MS) detection allows the interactions of metal complexes with biomolecules to be characterized. This technique has been used to provide new insights into the mode of action of the ruthenium‐based anticancer drug candidate indazolium [trans‐tetrachlorobis(1H‐indazole)ruthenate(III)] (KP1019). While the compound binds rapidly and efficiently to serum proteins, especially albumin, its reactivity towards the model DNA compound 2′‐deoxyguanosine 5′‐monophosphate (5′‐dGMP) is moderate.
ISSN:1612-1872
1612-1880
DOI:10.1002/cbdv.200890148