An optimized analysis of 2‐acetyl‐1‐pyrroline content diversity in the core collection of aromatic rice germplasm (Oryza sativa L.)

Background and objectives Aromatic rice constitutes a small but a special group of rice varieties, considered as the best in quality. A headspace solid‐phase micro‐extraction (HS‐SPME) method, combined with a gas chromatography–mass spectrometry (GC‐MS) assay, was developed to enable the rapid, simp...

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Bibliographic Details
Published in:Cereal chemistry 2019-07, Vol.96 (4), p.698-707
Main Authors: Xie, Li‐Hong, Tang, Shao‐Qing, Wei, Xiang‐Jin, Jiao, Gui‐Ai, Sheng, Zhong‐Hua, Hu, Pei‐Song
Format: Article
Language:English
Subjects:
gas chromatography–mass spectrometry
solid‐phase micro‐extraction
volatile compounds
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Summary:Background and objectives Aromatic rice constitutes a small but a special group of rice varieties, considered as the best in quality. A headspace solid‐phase micro‐extraction (HS‐SPME) method, combined with a gas chromatography–mass spectrometry (GC‐MS) assay, was developed to enable the rapid, simple, and reliable quantification of 2‐acetyl‐1‐pyrroline (2AP) in rice. Findings The most effective release of 2AP was obtained using a divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber and extracting brown flour or single whole brown grain at 80°C for 30 min. By the optimized protocol, the limit of detection (LOD) and limit of quantification (LOQ) for 2AP was 1.0 µg/kg of 2AP and 4.0 µg/kg of brown rice flour, respectively. The reproducibility and interday repeatability were
ISSN:0009-0352
1943-3638
DOI:10.1002/cche.10166