Solid-Phase Peptide Modification via Deaminative Photochemical Csp 3 -Csp 3 Bond Formation Using Katritzky Salts

• Published in: Chemistry : a European journal 2022-07, Vol.28 (39), p.e202201121
• Main Authors: Openy, Joseph, Amrahova, Gulshan, Chang, Jen-Yao, Noisier, Anaïs, Hart, Peter 't
• Format: Article
• Language: English
• Subjects: Amines - chemistry; Amino Acids - chemistry; Lysine; Peptides - chemistry; Salts - chemistry
• ISSN: 0947-6539; 1521-3765
• DOI: 10.1002/chem.202201121

Introduction of unnatural amino acids can significantly improve the binding affinity and stability of peptides. Commercial availability of such amino acids is limited, and their synthesis is a long and tedious process. We here describe a method that allows the functionalization of peptides directly on solid-support by converting lysine residues to Katritzky salts, and subjecting them to a photochemical Giese reaction under mild reaction conditions. The method avoids the need for amino acid synthesis and instead offers a late-stage modification route for rapid peptide diversification. While numerous modification approaches at the lysine amine have been described, this work provides the first example of deaminative functionalization of peptides at lysine. The two-step protocol is compatible with various substrates, lysine analogues, resins, and all proteinogenic amino acids. Finally, by leveraging solid-phase modification, this protocol facilitates the functionalization of longer peptides as was demonstrated using biologically relevant peptides of up to 15 amino acids.