Detection of resistance loci to English grain aphid in common wheat

• Published in: Crop science 2022-11, Vol.62 (6), p.2186-2196
• Main Authors: Zhankui, Zeng, Zhipeng, Han, Chunping, Wang, Zhenghong, Wang, Cheng, Guo, Liming, Wang, Li, Gao
• Format: Article
• Language: English
• ISSN: 0011-183X; 1435-0653
• DOI: 10.1002/csc2.20798

The English grain aphid (Sitobion avenae F.) causes significant yield and quality losses in wheat (Triticum aestivum L.) worldwide, and the use of insect‐resistant wheat cultivars reduces the loss level. The present study aimed to identify quantitative trait loci (QTL) and perform putative candidate gene evolutionary analysis for English grain aphid resistance in 261 F2:5 recombinant inbred lines (RILs) derived from the cross Amigo/XN1376. The RIL population and parents were genotyped with the Wheat 55K single nucleotide polymorphism (SNP) array. A total of 14,694 polymorphic SNPs were used to construct a genetic linkage map. One major effect of QTL was detected in F2:5 RILs and was located on chromosome 3DL, explained 19.33% phenotypic variance, temporarily designated as QSa3.haust‐3D. It was located in a 2.73 Mb, flanked by markers AX‐109429351 and AX‐110834100, from 550.25 to 552.98 Mb according to Chinese Spring reference genome. This region contained 39 annotated genes. Eight putative candidate genes for resistance to English grain aphid were identified among these genes. The QTL with closely linked SNP markers could be useful for marker‐assisted selection for resistance to English grain aphid for wheat breeding. Core Ideas A QTL controlling wheat resistance to English grain aphid was found on chromosome 3DL. QSa3.haust‐3D was located near the centromere of 3DL‐2, and the physical interval was 2.73 Mb. QSa3.haust‐3D was found that the loci may have evolved from Tausch's goatgrass (Aegilops tauschii).