Low bone mineral density in patients with type 1 diabetes: association with reduced expression of IGF1, IGF1R and TGF B 1 in peripheral blood mononuclear cells

• Published in: Diabetes/metabolism research and reviews 2016-09, Vol.32 (6), p.589-595
• Main Authors: de Souza, Karla Simone Costa, Ururahy, Marcela Abbott Galvão, da Costa Oliveira, Yonara Monique, Loureiro, Melina Bezerra, da Silva, Heglayne Pereira Vital, Bortolin, Raul Hernandes, Melo dos Santos, Fabricio, Luchessi, André Ducati, Neto, José Jorge Maciel, Arrais, Ricardo Fernando, Hirata, Rosario Dominguez Crespo, das Graças Almeida, Maria, Hirata, Mario Hiroyuki, de Rezende, Adriana Augusto
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Biomarkers - analysis; Blood Glucose - analysis; Bone Density; Case-Control Studies; Child; Diabetes Mellitus, Type 1 - metabolism; Diabetes Mellitus, Type 1 - pathology; Female; Follow-Up Studies; Glycated Hemoglobin A - analysis; Humans; IGF1; IGF1R; Insulin-Like Growth Factor I - genetics; Insulin-Like Growth Factor I - metabolism; Leukocytes, Mononuclear - metabolism; low BMD; Male; mRNA expression; Prognosis; Receptors, Somatomedin - genetics; Receptors, Somatomedin - metabolism; TGFB1; Transforming Growth Factor beta1 - genetics; Transforming Growth Factor beta1 - metabolism; type 1 diabetes
• ISSN: 1520-7552; 1520-7560
• DOI: 10.1002/dmrr.2772

Background The negative effects of type 1 diabetes (T1D) on growth factors of bone metabolism lead to a reduction in bone mineral density. This study aimed to evaluate the association between bone mineral density and insulin‐like growth factor 1 (IGF1), insulin‐like growth factor 1 receptor (IGF1R) and transforming growth factor beta 1 (TGFB1) expressions in children and adolescents with T1D. Moreover, the influences of age at diagnosis, time since diagnosis, glycaemic control and albuminuria on bone mineral density were investigated. Methods Eighty‐six T1D children/adolescents (T1D group) and ninety normoglycaemic controls (normoglycaemic group) were included. T1D patients were analysed as a whole and also in subsets of patients with good glycaemic control (glycated hemoglobin concentration ≤7.5%) and with poor glycaemic control (glycated hemoglobin concentration >7.5%). Bone mineral density was assessed by dual energy x‐ray absorptiometry. Glycaemic control, renal function and bone markers were also assessed. IGF1, IGF1R and TGFB1 expressions were determined in peripheral blood mononuclear cells by real‐time polymerase chain reaction. Results Patients with T1D showed low bone mineral density and poor glycaemic control. Serum total calcium and urinary albumin‐to‐creatinine ratio were higher in patients with poor glycaemic control compared to those with good glycemic control (p = 0.003 and p = 0.035, respectively). There was a reduction of IGF1, IGF1R and TGFB1 expressions in the T1D patients and in the subset with poor glycaemic control compared to normoglycaemic controls (p