Genetic linkage maps of the West African clawed frog Xenopus tropicalis

Amphibians, and particularly the African clawed frog Xenopus laevis, have been used for more than a century as models of vertebrate embryonic development. However, in many cases, elucidation of developmental functions of specific gene sequences could be severely impeded, because X. laevis is a tetra...

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Bibliographic Details
Published in:Developmental dynamics 2003-01, Vol.226 (1), p.99-102
Main Authors: Kochan, Kelli J., Wright, David A., Schroeder, Lisa J., Shen, Jianjun, Morizot, Donald C.
Format: Article
Language:English
Subjects:
AFLP
Africa
Animals
Chromosome Mapping
Cloning, Molecular
gene map
Genetic Linkage
Genetic Markers
isozymes
Models, Genetic
Polymorphism, Genetic
Protein Isoforms
Xenopus - genetics
Xenopus tropicalis
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Summary:Amphibians, and particularly the African clawed frog Xenopus laevis, have been used for more than a century as models of vertebrate embryonic development. However, in many cases, elucidation of developmental functions of specific gene sequences could be severely impeded, because X. laevis is a tetraploid species, with multiple functional copies of many genes of interest. Recent studies have shifted focus to the West African or tropical clawed frog, X. tropicalis, the only known diploid species of the genus Xenopus. Here, we present two preliminary linkage maps, constructed by analysis of joint segregation of amplified fragment length polymorphism (AFLP) markers in a X. tropicalis interstrain hybrid. A total of 53 markers, including 51 AFLP markers and 2 isozyme markers, are presently assigned to 13 multipoint linkage groups on a map of the maternal strain, whereas 9 AFLP markers from the paternal strain are assigned to 3 linkage groups on a separate map. A dense genetic linkage map is essential in mapping new developmental mutants and determining their sequences by positional cloning. © 2002 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.10193