A novel POZ/zinc finger protein, champignon, interferes with gastrulation movements in Xenopus

We have cloned a novel krüppel‐like transcription factor of Xenopus that encodes POZ/zinc finger protein by expression cloning. Overexpression of mRNA resulted in interference with gastrulation. Because the injected embryo looks like a mushroom in appearance at the neurula stage, we have named this...

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Bibliographic Details
Published in:Developmental dynamics 2001-05, Vol.221 (1), p.14-25
Main Authors: Goto, Toshiyasu, Hasegawa, Kouichi, Kinoshita, Tsutomu, Kubota, Hiroshi Y.
Format: Article
Language:English
Subjects:
Animals
champignon
convergent extension
DNA-Binding Proteins - genetics
Dose-Response Relationship, Drug
Embryo, Nonmammalian - embryology
Embryo, Nonmammalian - physiology
Embryo, Nonmammalian - ultrastructure
Female
Gastrula - drug effects
Gastrula - physiology
gastrulation
Gene Expression Regulation, Developmental - physiology
Kruppel-Like Transcription Factors
Microinjections
Microscopy, Electron, Scanning
Molecular Sequence Data
Movement
Mutagenesis - physiology
POZ domain
Repressor Proteins
Sequence Homology, Amino Acid
transcription factor
Transcription Factors - genetics
Transcription Factors - pharmacology
Xenopus
Xenopus laevis
Xenopus Proteins
zinc finger domain
Zinc Fingers - genetics
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Summary:We have cloned a novel krüppel‐like transcription factor of Xenopus that encodes POZ/zinc finger protein by expression cloning. Overexpression of mRNA resulted in interference with gastrulation. Because the injected embryo looks like a mushroom in appearance at the neurula stage, we have named this gene champignon (cpg). In cpg‐injected embryos, the blastopore appeared normally, but regressed thereafter. The injected embryos then elongated along the primary dorsoventral axis during the tailbud stage. Histologic sections and reverse transcription‐polymerase chain reaction analysis showed that cpg had no effect on the cell differentiation. The animal pole region of cpg‐injected embryos was thick during the gastrula stage, and mesodermal cells remained in the marginal zone. Furthermore, neither Keller‐sandwich explants nor activin‐treated animal cap explants excised from cpg‐injected embryos elongated. These results suggest that cpg acts as a potent inhibitor of cell migration and cell intercalation during gastrulation. © 2001 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.1121