Transcriptional regulation of eater gene expression in Drosophila blood cells

Eater is a transmembrane protein that mediates phagocytosis in Drosophila. eater was identified in a microarray analysis of genes downregulated in S2 cells, in which Serpent had been knocked down by RNAi. The gene was shown to be expressed predominantly in plasmatocytes after embryonic development....

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Bibliographic Details
Published in:Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2012-01, Vol.50 (1), p.41-49
Main Authors: Kroeger Jr, Paul T., Tokusumi, Tsuyoshi, Schulz, Robert A.
Format: Article
Language:English
Subjects:
Animals
Binding Sites - genetics
Blood Cells - metabolism
Cell Differentiation
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Drosophila
Drosophila - cytology
Drosophila - embryology
Drosophila - genetics
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Electrophoretic Mobility Shift Assay - methods
enhancer analysis
Enhancer Elements, Genetic
GATA Transcription Factors - genetics
GATA Transcription Factors - metabolism
Gene Expression Regulation, Developmental
Hematopoiesis
Hemocytes - cytology
Hemocytes - metabolism
Membrane Proteins - genetics
Membrane Proteins - metabolism
Multigene Family
Mutagenesis, Site-Directed
plasmatocyte
Receptors, Cell Surface - genetics
Receptors, Cell Surface - metabolism
RNA Interference
Serpent
Transcription, Genetic
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Summary:Eater is a transmembrane protein that mediates phagocytosis in Drosophila. eater was identified in a microarray analysis of genes downregulated in S2 cells, in which Serpent had been knocked down by RNAi. The gene was shown to be expressed predominantly in plasmatocytes after embryonic development. We have extensively analyzed the transcriptional enhancer controlling eater expression with the following findings: the enhancer reproduces the plasmatocyte expression pattern of the gene as verified by anti‐P1 antibody staining and a 526‐basepair DNA region is active in lymph gland and hemolymph plasmatocytes. This DNA contains several GATA elements that serve as putative‐binding sites for Serpent. Site‐directed mutagenesis of two of these GATA sites abolishes eater expression in both lymph gland and hemolymph plasmatocytes. This suggests that Serpent regulates eater expression by binding these GATA sites, which was confirmed by gel shift analysis. These analyses allowed us to use eater‐Gal4 to force plasmatocyte to lamellocyte differentiation. genesis 50:41–49, 2012. © 2011 Wiley Periodicals, Inc.
ISSN:1526-954X
1526-968X
DOI:10.1002/dvg.20787