Generation and characterization of a tamoxifen-inducible Vsx1-CreER T2 line to target V2 interneurons in the mouse developing spinal cord

In the spinal cord, ventral interneurons regulate the activity of motor neurons, thereby controlling motor activities including locomotion. Interneurons arise during embryonic development from distinct progenitor domains orderly distributed along the dorso-ventral axis of the neural tube. The p2 pro...

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Published in:Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2021-08, Vol.59 (7-8), p.e23435
Main Authors: Baudouin, Charlotte, Pelosi, Barbara, Courtoy, Guillaume E, Achouri, Younes, Clotman, Frédéric
Format: Article
Language:English
Subjects:
Animals
Cell Lineage
Eye Proteins - genetics
Eye Proteins - metabolism
Gene Targeting - methods
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Integrases - genetics
Integrases - metabolism
Interneurons - cytology
Interneurons - metabolism
Mice
Mice, Inbred C57BL
Neurogenesis
Spinal Cord - cytology
Spinal Cord - embryology
Spinal Cord - metabolism
Tamoxifen - pharmacology
Transcriptional Activation - drug effects
Transgenes
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Summary:In the spinal cord, ventral interneurons regulate the activity of motor neurons, thereby controlling motor activities including locomotion. Interneurons arise during embryonic development from distinct progenitor domains orderly distributed along the dorso-ventral axis of the neural tube. The p2 progenitor domain generates at least five V2 interneuron populations. However, identification and characterization of all V2 populations remain currently incomplete and the mechanisms that control their development remain only partly understood. In this study, we report the generation of a Vsx1-CreER BAC transgenic mouse line that drives CreER recombinase expression mimicking endogenous Vsx1 expression pattern in the developing spinal cord. We showed that the Vsx1-CreER transgene can mediate recombination in V2 precursors with a high efficacy and specificity. Lineage tracing demonstrated that all the V2 interneurons in the mouse developing spinal cord derive from cells expressing Vsx1. Finally, we confirmed that V2 precursors generate additional V2 populations that are not characterized yet. Thus, the Vsx1-CreER line described here is a useful genetic tool for lineage tracing and for functional studies of the mouse spinal V2 interneurons.
ISSN:1526-954X
1526-968X
DOI:10.1002/dvg.23435