Thermomyces lanuginosus lipase catalyzed transesterification of sterols by methyl oleate

Plant steryl and stanyl esters are effective in lowering plasma cholesterol concentration by inhibiting the absorption of cholesterol from small intestine. They are, therefore, used as constituents of special margarines, which are commercially available as functional foods with the ability to reduce...

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Bibliographic Details
Published in:European journal of lipid science and technology 2003-10, Vol.105 (10), p.624-626
Main Authors: Weber, Nikolaus, Weitkamp, Petra, Mukherjee, Kumar D.
Format: Article
Language:English
Subjects:
5α-cholestanol oleate
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
cholesteryl oleate
enzymatic preparation
Fat industries
Food industries
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
sitostanyl oleate
Thermomyces lanuginosus lipase
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Summary:Plant steryl and stanyl esters are effective in lowering plasma cholesterol concentration by inhibiting the absorption of cholesterol from small intestine. They are, therefore, used as constituents of special margarines, which are commercially available as functional foods with the ability to reduce blood cholesterol levels. An enzymatic method has been developed for the preparation of oleic acid esters of sterols and stanols in high yield by transesterification of methyl oleate with the 3‐hydroxy group in vacuo (20‐40 hPa) at moderate temperature (80 °C) using immobilized lipase preparation from Thermomyces lanuginosus (Lipozyme TL IM®) as biocatalyst. Neither an organic solvent nor a drying agent are required. Cholesterol and stanols such as 5α‐cholestanol and sitostanol have been converted at similar rates and in near‐quantitative yields (>95%) to the corresponding oleic acid esters.
ISSN:1438-7697
1438-9312
DOI:10.1002/ejlt.200300825