Oxidation products of free polyunsaturated fatty acids in wheat varieties

Oxygenated fatty acids (oxylipins) are secondary metabolites of polyunsaturated fatty acids (PUFA). Here, we present a novel high-performance liquid chromatograpic separation on a reversed-phase column (RP-HPLC) coupled with electrospray ionization-tandem mass spectrometry (ESI-MS/MS) for the determ...

Full description

Saved in:
Bibliographic Details
Published in:European journal of lipid science and technology 2009-07, Vol.111 (7), p.715-722
Main Authors: Levandi, Tuuli, Püssa, Tõnu, Vaher, Merike, Toomik, Peeter, Kaljurand, Mihkel
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cereal and baking product industries
Fat industries
Food industries
Fundamental and applied biological sciences. Psychology
HPLC-MS/MS
Iso-LTX-diol
LTX-diol
Oxylipins
PCA
Wheat
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Oxygenated fatty acids (oxylipins) are secondary metabolites of polyunsaturated fatty acids (PUFA). Here, we present a novel high-performance liquid chromatograpic separation on a reversed-phase column (RP-HPLC) coupled with electrospray ionization-tandem mass spectrometry (ESI-MS/MS) for the determination of various (per)oxidation products of linoleic (cis,cis-9,12-octadecadienoic) acid in eight different varieties (four spring and four winter varieties) of wheat (Triticum aestivum). The procedure includes extraction of oxylipins, chromatograpic separation using a linear gradient of aqueous formic acid and acetonitrile, with subsequent identification of compounds by MS/MS. Among the identified oxylipins, leukotoxin (LTX)-diol and its isomer (iso-LTX-diol) are known as potentially toxic substances. The obtained data was used further for comparison of different wheat varieties by principal component analysis (PCA). From the results of PCA, differences can be observed in the patterns of wheat varieties.
ISSN:1438-7697
1438-9312
DOI:10.1002/ejlt.200800286