A Microbial Biosensor for p-Nitrophenol Using Arthrobacter Sp

This article reports the construction, optimization of performance variables and analytical characterization of a sensitive and selective microbial amperometric biosensor for measurement of p‐nitrophenol (PNP), a U.S. Environmental Agency priority pollutant. The biosensor consisted of PNP‐degrading/...

Full description

Saved in:
Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2003-08, Vol.15 (14), p.1160-1164
Main Authors: Lei, Yu, Mulchandani, Priti, Chen, Wilfred, Wang, Joseph, Mulchandani, Ashok
Format: Article
Language:English
Subjects:
4-Nitrophenol
Arthrobacter sp
Biosensor
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This article reports the construction, optimization of performance variables and analytical characterization of a sensitive and selective microbial amperometric biosensor for measurement of p‐nitrophenol (PNP), a U.S. Environmental Agency priority pollutant. The biosensor consisted of PNP‐degrading/oxidizing bacteria Arthrobacter sp. JS443 as biological sensing element and a dissolved oxygen electrode as the transducer. The best sensitivity and response time were obtained using a sensor constructed with 1.2 mg dry wt. of cells and operating in pH 7.5, 50 mM citrate‐phosphate buffer. Using these conditions, the biosensor was able to measure as low as 28 ppb (0.2 μM) of PNP selectively without interference from structurally similar compounds, such as phenol, nitrophenols and chlorophenols. The service life of the microbial biosensor is around 5 days when stored in the operating buffer at 4 °C. The applicability to lake water is demonstrated.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.200390141