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Electrochemistry and Electrocatalysis of a Nafion/Nano‐CaCO 3 /Hb Film Modified Carbon Ionic Liquid Electrode Using BMIMPF 6 as Binder
In this paper a room temperature ionic liquid 1‐butyl‐3‐methylimidazolium hexafluorophosphate (BMIMPF 6 ) was used as binder for the construction of carbon ionic liquid electrode (CILE) and a new electrochemical biosensor was developed for determination of H 2 O 2 by immobilization of hemoglobin (Hb...
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Published in: | Electroanalysis (New York, N.Y.) N.Y.), 2007-07, Vol.19 (13), p.1368-1374 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | In this paper a room temperature ionic liquid 1‐butyl‐3‐methylimidazolium hexafluorophosphate (BMIMPF
6
) was used as binder for the construction of carbon ionic liquid electrode (CILE) and a new electrochemical biosensor was developed for determination of H
2
O
2
by immobilization of hemoglobin (Hb) in the composite film of Nafion/nano‐CaCO
3
on the surface of CILE. The Hb modified electrode showed a pair of well‐defined, quasi‐reversible redox peaks with
E
pa
and
E
pc
as −0.265 V and −0.470 V (vs. SCE). The formal potential (
E
°′) was got by the midpoint of
E
pa
and
E
pc
as −0.368 V, which was the characteristic of Hb Fe(III)/Fe(II) redox couples. The peak to peak separation was 205 mV in pH 7.0 Britton–Robinson (B–R) buffer solution at the scan rate of 100 mV/s. The direct electrochemistry of Hb in the film was carefully investigated and the electrochemical parameters of Hb on the modified electrode were calculated as
α
=0.487 and
k
s
=0.128 s
−1
. The Nafion/nano‐CaCO
3
/Hb film electrode showed good electrocatalysis to the reduction of H
2
O
2
in the linear range from 8.0 to 240.0 μmol/L and the detection limit as 5.0 μmol/L (3
σ
). The apparent Michaelis–Menten constant (
K
M
app
) was estimated to be 65.7 μmol/L. UV‐vis absorption spectroscopy and FT‐IR spectroscopy showed that Hb in the Nafion/nano‐CaCO
3
composite film could retain its native structure. |
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ISSN: | 1040-0397 1521-4109 |
DOI: | 10.1002/elan.200703859 |