A Sensor for Serotonin and Dopamine Detection in Cancer Cells Line Based on the Conducting Polymer−Pd Complex Composite

• Published in: Electroanalysis (New York, N.Y.) N.Y.), 2020-03, Vol.32 (3), p.520-527
• Main Authors: Chung, Saeromi, Akhtar, Mahmood H., Benboudiaf, A., Park, Deog‐Su, Shim, Yoon‐Bo
• Format: Article
• Language: English
• Subjects: Breast cancer cell; Conducting polymer; Electrochemical sensor; Pd complex; Serotonin
• ISSN: 1040-0397; 1521-4109
• DOI: 10.1002/elan.201900568

An electrochemical sensor based on the conducting polymer composite with a palladium complex (Pd(C2H4N2S2)2) was developed for the detection of serotonin and dopamine simultaneously in the breast cancer cell and human plasma samples. The proposed sensor was fabricated using the Pd(C2H4N2S2)2 complex‐anchored poly2,2 : 5,2‐terthiophene‐3‐(p‐benzoic acid) (pTBA) layer on the AuNPs decorated reduced graphene oxide (AuNPs@rGO) substrate, which revealed the enhanced anodic current of the target species. The sensor probe was characterized by electrochemical and surface analysis methods. The experimental parameters affecting the sensor performance were optimized, in terms of AuNPs@rGO concentration, the number of electropolymerization cycle for pTBA, immobilization time of Pd(C2H4N2S2)2, and pH. The dynamic ranges for serotonin and dopamine were obtained from 0.02 to 200 μM, and from 0.1 to 200 μM with the detection limit of 2.5, and 24.0 nM, respectively. The reliability of proposed sensor was evaluated using cancer cell lines for the clinical applications.