Highly Sensitive Impedimetric Biosensor Based on Thermolysin Immobilized on a GCE Modified with AuNP‐decorated Graphene for the Detection of Ochratoxin A

The fabrication of a thermolysin‐based biosensor capable of detecting ochratoxin A (OTA) from food samples is described. The electrochemical deposition of calcium cross‐linked cellulose film (CCLC) and gold nanoparticles (AuNPs) on graphene (GR) for modification of a glassy carbon electrode (GCE) is...

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Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2021-01, Vol.33 (1), p.136-145
Main Authors: Zinoubi, Khaoula, Chrouda, Amani, Soltane, Raya, Al‐Ghamdi, Youssef O., Garallah Almalki, Sami, Osman, Gamal, Barhoumi, Houcine, Jaffrezic Renault, Nicole
Format: Article
Language:English
Subjects:
Biosensor
Electrochemical impedance spectroscopy
Ochratoxin A
Thermolysin
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Summary:The fabrication of a thermolysin‐based biosensor capable of detecting ochratoxin A (OTA) from food samples is described. The electrochemical deposition of calcium cross‐linked cellulose film (CCLC) and gold nanoparticles (AuNPs) on graphene (GR) for modification of a glassy carbon electrode (GCE) is the first step. Then the thermolysin (TLN) enzyme in a polyvinyl alcohol (PVA)/polyethylenimine (PEI) matrix is immobilized. The impedimetric biosensor response is linear from 0.2 nM to 100 nM with a detection limit of 0.2 nM. The obtained stable and reproducible biosensor is then applied for the detection of OTA in spiked extracts from coffee beans.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.202060247