Quality-assurance study of marine lipid-class determination using chromarod/iatroscan® thin-layer chromatography-flame ionization detector

An Iatroscan® thin‐layer chromatography–flame ionization detector has been utilized to quantify lipid classes in marine samples. This method was evaluated relative to established quality‐assurance (QA) procedures used for the gas chromatographic analysis of PCBs. A method for extracting and analyzin...

Full description

Saved in:
Bibliographic Details
Published in:Environmental toxicology and chemistry 2000-09, Vol.19 (9), p.2189-2197
Main Authors: Bergen, Barbara J., Quinn, James G., Parrish, Christopher C.
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Applied ecology
Biological and medical sciences
Chromatography
Ecotoxicology, biological effects of pollution
Fundamental and applied biological sciences. Psychology
Iatroscan
Ionization detector
Marine lipids
Quality assurance
Techniques
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An Iatroscan® thin‐layer chromatography–flame ionization detector has been utilized to quantify lipid classes in marine samples. This method was evaluated relative to established quality‐assurance (QA) procedures used for the gas chromatographic analysis of PCBs. A method for extracting and analyzing eight major lipid classes in the ribbed mussel (Guekensia demissus) was developed. The analytical method met the QA criteria prescribed for consistent external calibrations, low blanks, complete extraction of all lipid classes, and precise replicate analysis. Matrix and blank spikes were satisfactorily recovered (50‐130%), provided that the samples contained a large enough mass (>4% dry weight) of total lipids to overcome the absorption of polar lipids on glassware. The use of frozen mussel homogenate as a standard reference material was not possible because of lipid degradation, particularly of triacylglycerols and phospholipids. Also, total lipids measured gravimetrically significantly decreased in frozen samples, which could influence bioaccumulation predictions. A laboratory intercalibration was performed using a mussel homogenate and chloroform extract, which verified the accuracy of the method and the lipid‐class identification. Characterizing the structure of one class of polar lipids, the acetone mobile polar lipid (AMPL), showed that it contained no ester linkages or free/sterically unhindered ‐OH groups; however, the AMPL did contain an ether linkage.
ISSN:0730-7268
1552-8618
DOI:10.1002/etc.5620190907