Effects of nonylphenol, bisphenol a, and their mixture on the viviparous swordtail fish (Xiphophorus helleri)

• Published in: Environmental toxicology and chemistry 2001-04, Vol.20 (4), p.787-795
• Main Authors: Kwak, Hyeong-Il, Bae, Mi-Ok, Lee, Moon-Han, Lee, Yong-Soon, Lee, Bum-Joon, Kang, Kyung-Sun, Chae, Chan-Hee, Sung, Ha-Jung, Shin, Jin-Sup, Kim, Jin-Hwa, Mar, Woong-Cheon, Sheen, Yhun-Yong, Cho, Myung-Haing
• Format: Article
• Language: English
• Subjects: Agnatha. Pisces; Air Pollutants, Occupational - toxicity; Animal, plant and microbial ecology; Animals; Apoptosis - drug effects; Applied ecology; Benzhydryl Compounds; Biological and medical sciences; Bisphenol A; Cyprinodontiformes - growth & development; Cyprinodontiformes - physiology; Drug Interactions; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on vertebrates; Endocrine disruptor; Flow Cytometry; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; In Situ Nick-End Labeling; Male; Models, Biological; Necrosis; Nonylphenol; Phenols - toxicity; Reproduction - drug effects; RNA, Messenger - genetics; Seminiferous Tubules - drug effects; Seminiferous Tubules - pathology; Swordtail; Testis - drug effects; Testis - pathology; Transcription, Genetic - drug effects; Vitellogenesis; Vitellogenins - genetics; Water Pollutants, Chemical - toxicity
• ISSN: 0730-7268; 1552-8618
• DOI: 10.1002/etc.5620200414

A number of fish species have been used for studies on endocrine disrupting chemicals (EDCs). However, despite the widespread use of oviparous fish, relatively little attention has been given to viviparous species. This study investigated the effects of EDCs in a viviparous fish and examined the possible usefulness of the fish as an alternative model for the studies on EDCs. Swordtails (Xiphophorus helleri) were exposed to nonylphenol (NP), bisphenol A (BPA), and their mixture. Both short‐term (3‐d) and relatively long‐term (60‐d) exposures were carried out using adult male and 30‐d‐old juvenile fish, respectively. Following the short‐term exposure, both NP and BPA caused vitellogenin mRNA expression. Flow cytometric analysis and terminal deox‐ynucleotidyl transferase assay on the testes of treated fish indicated reproductive damage. Histopathological analysis found degenerative and necrotic cells in seminiferous tubules following the exposure to 100 ppb NP. The testes with lesions were also associated with highly suppressed spermatogenesis. Following the long‐term exposure, both NP and BPA exposures significantly affected the growth of swordtails. In all cases, the results showed that the mixture was always more potent than a single chemical and that swordtail fish can be a useful model for the study of endocrine disruptors.