Phytochemical Profiling and Pharmacological Evaluation of Methanolic Leaf Extract of C. digyna for Cytotoxic, Anti‐inflammatory, Antioxidant, Antiarthritic, and Analgesic Activities

ABSTRACT Caesalpinia digyna (Family: Fabaceae) is traditionally used in Ayurvedic medicine for various medicinal purposes, including as a treatment for wounds, leprosy, skin diseases, fever, diabetes, etc. Although the root and stem of this plant have a significant medicinal value, there was little...

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Published in:Food science & nutrition 2024-11, Vol.12 (12), p.10231-10241
Main Authors: Fatema, Kanij, Mia, Md. Abdur Rashid, Nipun, Tanzina Sharmin, Hossen, S. M. Moazzem
Format: Article
Language:English
Subjects:
Acetic acid
acetic acid‐induced writhing
Alternative medicine
Analgesics
Anti-inflammatory agents
Antioxidants
Bioassays
Biopolymer denaturation
Blood levels
brine shrimp lethality bioassay
Caesalpinia digyna
Carrageenan
carrageenan‐induced paw edema
Cytotoxicity
Diabetes mellitus
DPPH assay
Edema
Erythrocytes
Free radicals
Herbal medicine
HRBC membrane lysis
Hypotonicity
Immunology
In vitro methods and tests
In vivo methods and tests
Inflammation
Leaves
Lethality
Lysis
Medicinal plants
Nonsteroidal anti-inflammatory drugs
Original
Phytochemicals
Plant extracts
Plants (botany)
Protein denaturation
Scavenging
Skin diseases
tail immersion
Vincristine
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Mia, Md. Abdur Rashid
Nipun, Tanzina Sharmin
Hossen, S. M. Moazzem
description ABSTRACT Caesalpinia digyna (Family: Fabaceae) is traditionally used in Ayurvedic medicine for various medicinal purposes, including as a treatment for wounds, leprosy, skin diseases, fever, diabetes, etc. Although the root and stem of this plant have a significant medicinal value, there was little research on the leaves of this plant. This study aimed to investigate the qualitative phytochemical profile and evaluate the in vitro cytotoxic, anti‐inflammatory, antioxidant, and antiarthritic activities, as well as the in vivo anti‐inflammatory and analgesic activities, of C. digyna leaf extract. The methanolic extract of C. digyna leaves was prepared using an ultrasonic‐assisted extraction process. In vitro and in vivo anti‐inflammatory activities were evaluated using the hypotonicity‐induced hemolysis and carrageenan‐induced paw edema methods, respectively. Additionally, the extract was assessed for in vitro DPPH (1, 1‐diphenyl‐2‐picrylhydrazyl) free radical scavenging, antiarthritic (protein denaturation), and in vivo analgesic (acetic acid‐induced writhing and tail immersion) activities. Brine shrimp lethality bioassay (BSLB) showed moderate cytotoxic activity (LC50 = 2.25 μg/mL) compared with the standard vincristine sulfate (LC50 = 1.61 μg/mL). In vitro, anti‐inflammatory activity exhibited 85.13% (IC50 value = 2.51 μg/mL) inhibition of Human Red Blood Cell (HRBC) membrane lysis at a concentration of 2000 μg/mL whereas in vivo anti‐inflammatory study exerted its maximum effect (p 
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Abdur Rashid ; Nipun, Tanzina Sharmin ; Hossen, S. M. Moazzem</creator><creatorcontrib>Fatema, Kanij ; Mia, Md. Abdur Rashid ; Nipun, Tanzina Sharmin ; Hossen, S. M. Moazzem</creatorcontrib><description>ABSTRACT Caesalpinia digyna (Family: Fabaceae) is traditionally used in Ayurvedic medicine for various medicinal purposes, including as a treatment for wounds, leprosy, skin diseases, fever, diabetes, etc. Although the root and stem of this plant have a significant medicinal value, there was little research on the leaves of this plant. This study aimed to investigate the qualitative phytochemical profile and evaluate the in vitro cytotoxic, anti‐inflammatory, antioxidant, and antiarthritic activities, as well as the in vivo anti‐inflammatory and analgesic activities, of C. digyna leaf extract. The methanolic extract of C. digyna leaves was prepared using an ultrasonic‐assisted extraction process. In vitro and in vivo anti‐inflammatory activities were evaluated using the hypotonicity‐induced hemolysis and carrageenan‐induced paw edema methods, respectively. Additionally, the extract was assessed for in vitro DPPH (1, 1‐diphenyl‐2‐picrylhydrazyl) free radical scavenging, antiarthritic (protein denaturation), and in vivo analgesic (acetic acid‐induced writhing and tail immersion) activities. Brine shrimp lethality bioassay (BSLB) showed moderate cytotoxic activity (LC50 = 2.25 μg/mL) compared with the standard vincristine sulfate (LC50 = 1.61 μg/mL). In vitro, anti‐inflammatory activity exhibited 85.13% (IC50 value = 2.51 μg/mL) inhibition of Human Red Blood Cell (HRBC) membrane lysis at a concentration of 2000 μg/mL whereas in vivo anti‐inflammatory study exerted its maximum effect (p &lt; 0.05) at 400 mg/kg bw dose. This extract also showed significant antioxidant (IC50 = 0.218 μg/mL), antiarthritic (83.61% inhibition) activity, and moderate analgesic effect (p &lt; 0.05) in both methods. These research findings indicated that C. digyna leaves extract has potent antioxidant, analgesic, and anti‐inflammatory effects which can be used as a supplementary medication for inflammatory pain‐relieving factors. In future, finding the mechanism involved in these effects could have significant impact on clinical science. DPPH: 1, 1‐diphenyl 2‐picrylhyorazyl, HRBC: human red blood cell.</description><identifier>ISSN: 2048-7177</identifier><identifier>EISSN: 2048-7177</identifier><identifier>DOI: 10.1002/fsn3.4504</identifier><language>eng</language><publisher>Malden, Massachusetts: John Wiley &amp; Sons, Inc</publisher><subject>Acetic acid ; acetic acid‐induced writhing ; Alternative medicine ; Analgesics ; Anti-inflammatory agents ; Antioxidants ; Bioassays ; Biopolymer denaturation ; Blood levels ; brine shrimp lethality bioassay ; Caesalpinia digyna ; Carrageenan ; carrageenan‐induced paw edema ; Cytotoxicity ; Diabetes mellitus ; DPPH assay ; Edema ; Erythrocytes ; Free radicals ; Herbal medicine ; HRBC membrane lysis ; Hypotonicity ; Immunology ; In vitro methods and tests ; In vivo methods and tests ; Inflammation ; Leaves ; Lethality ; Lysis ; Medicinal plants ; Nonsteroidal anti-inflammatory drugs ; Original ; Phytochemicals ; Plant extracts ; Plants (botany) ; Protein denaturation ; Scavenging ; Skin diseases ; tail immersion ; Vincristine</subject><ispartof>Food science &amp; nutrition, 2024-11, Vol.12 (12), p.10231-10241</ispartof><rights>2024 The Author(s). published by Wiley Periodicals LLC.</rights><rights>2024. 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Abdur Rashid</creatorcontrib><creatorcontrib>Nipun, Tanzina Sharmin</creatorcontrib><creatorcontrib>Hossen, S. M. Moazzem</creatorcontrib><title>Phytochemical Profiling and Pharmacological Evaluation of Methanolic Leaf Extract of C. digyna for Cytotoxic, Anti‐inflammatory, Antioxidant, Antiarthritic, and Analgesic Activities</title><title>Food science &amp; nutrition</title><description>ABSTRACT Caesalpinia digyna (Family: Fabaceae) is traditionally used in Ayurvedic medicine for various medicinal purposes, including as a treatment for wounds, leprosy, skin diseases, fever, diabetes, etc. Although the root and stem of this plant have a significant medicinal value, there was little research on the leaves of this plant. This study aimed to investigate the qualitative phytochemical profile and evaluate the in vitro cytotoxic, anti‐inflammatory, antioxidant, and antiarthritic activities, as well as the in vivo anti‐inflammatory and analgesic activities, of C. digyna leaf extract. The methanolic extract of C. digyna leaves was prepared using an ultrasonic‐assisted extraction process. In vitro and in vivo anti‐inflammatory activities were evaluated using the hypotonicity‐induced hemolysis and carrageenan‐induced paw edema methods, respectively. Additionally, the extract was assessed for in vitro DPPH (1, 1‐diphenyl‐2‐picrylhydrazyl) free radical scavenging, antiarthritic (protein denaturation), and in vivo analgesic (acetic acid‐induced writhing and tail immersion) activities. Brine shrimp lethality bioassay (BSLB) showed moderate cytotoxic activity (LC50 = 2.25 μg/mL) compared with the standard vincristine sulfate (LC50 = 1.61 μg/mL). In vitro, anti‐inflammatory activity exhibited 85.13% (IC50 value = 2.51 μg/mL) inhibition of Human Red Blood Cell (HRBC) membrane lysis at a concentration of 2000 μg/mL whereas in vivo anti‐inflammatory study exerted its maximum effect (p &lt; 0.05) at 400 mg/kg bw dose. This extract also showed significant antioxidant (IC50 = 0.218 μg/mL), antiarthritic (83.61% inhibition) activity, and moderate analgesic effect (p &lt; 0.05) in both methods. These research findings indicated that C. digyna leaves extract has potent antioxidant, analgesic, and anti‐inflammatory effects which can be used as a supplementary medication for inflammatory pain‐relieving factors. In future, finding the mechanism involved in these effects could have significant impact on clinical science. 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Moazzem</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2784-ddbb29f37e0dbf0c788a7d65c494b56fe727b4e75f08dc75e1b8c6962b2908ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acetic acid</topic><topic>acetic acid‐induced writhing</topic><topic>Alternative medicine</topic><topic>Analgesics</topic><topic>Anti-inflammatory agents</topic><topic>Antioxidants</topic><topic>Bioassays</topic><topic>Biopolymer denaturation</topic><topic>Blood levels</topic><topic>brine shrimp lethality bioassay</topic><topic>Caesalpinia digyna</topic><topic>Carrageenan</topic><topic>carrageenan‐induced paw edema</topic><topic>Cytotoxicity</topic><topic>Diabetes mellitus</topic><topic>DPPH assay</topic><topic>Edema</topic><topic>Erythrocytes</topic><topic>Free radicals</topic><topic>Herbal medicine</topic><topic>HRBC membrane lysis</topic><topic>Hypotonicity</topic><topic>Immunology</topic><topic>In vitro methods and tests</topic><topic>In vivo methods and tests</topic><topic>Inflammation</topic><topic>Leaves</topic><topic>Lethality</topic><topic>Lysis</topic><topic>Medicinal plants</topic><topic>Nonsteroidal anti-inflammatory drugs</topic><topic>Original</topic><topic>Phytochemicals</topic><topic>Plant extracts</topic><topic>Plants (botany)</topic><topic>Protein denaturation</topic><topic>Scavenging</topic><topic>Skin diseases</topic><topic>tail immersion</topic><topic>Vincristine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fatema, Kanij</creatorcontrib><creatorcontrib>Mia, Md. 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Abdur Rashid</au><au>Nipun, Tanzina Sharmin</au><au>Hossen, S. M. Moazzem</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phytochemical Profiling and Pharmacological Evaluation of Methanolic Leaf Extract of C. digyna for Cytotoxic, Anti‐inflammatory, Antioxidant, Antiarthritic, and Analgesic Activities</atitle><jtitle>Food science &amp; nutrition</jtitle><date>2024-11-04</date><risdate>2024</risdate><volume>12</volume><issue>12</issue><spage>10231</spage><epage>10241</epage><pages>10231-10241</pages><issn>2048-7177</issn><eissn>2048-7177</eissn><abstract>ABSTRACT Caesalpinia digyna (Family: Fabaceae) is traditionally used in Ayurvedic medicine for various medicinal purposes, including as a treatment for wounds, leprosy, skin diseases, fever, diabetes, etc. Although the root and stem of this plant have a significant medicinal value, there was little research on the leaves of this plant. This study aimed to investigate the qualitative phytochemical profile and evaluate the in vitro cytotoxic, anti‐inflammatory, antioxidant, and antiarthritic activities, as well as the in vivo anti‐inflammatory and analgesic activities, of C. digyna leaf extract. The methanolic extract of C. digyna leaves was prepared using an ultrasonic‐assisted extraction process. In vitro and in vivo anti‐inflammatory activities were evaluated using the hypotonicity‐induced hemolysis and carrageenan‐induced paw edema methods, respectively. Additionally, the extract was assessed for in vitro DPPH (1, 1‐diphenyl‐2‐picrylhydrazyl) free radical scavenging, antiarthritic (protein denaturation), and in vivo analgesic (acetic acid‐induced writhing and tail immersion) activities. Brine shrimp lethality bioassay (BSLB) showed moderate cytotoxic activity (LC50 = 2.25 μg/mL) compared with the standard vincristine sulfate (LC50 = 1.61 μg/mL). In vitro, anti‐inflammatory activity exhibited 85.13% (IC50 value = 2.51 μg/mL) inhibition of Human Red Blood Cell (HRBC) membrane lysis at a concentration of 2000 μg/mL whereas in vivo anti‐inflammatory study exerted its maximum effect (p &lt; 0.05) at 400 mg/kg bw dose. This extract also showed significant antioxidant (IC50 = 0.218 μg/mL), antiarthritic (83.61% inhibition) activity, and moderate analgesic effect (p &lt; 0.05) in both methods. These research findings indicated that C. digyna leaves extract has potent antioxidant, analgesic, and anti‐inflammatory effects which can be used as a supplementary medication for inflammatory pain‐relieving factors. In future, finding the mechanism involved in these effects could have significant impact on clinical science. DPPH: 1, 1‐diphenyl 2‐picrylhyorazyl, HRBC: human red blood cell.</abstract><cop>Malden, Massachusetts</cop><pub>John Wiley &amp; Sons, Inc</pub><doi>10.1002/fsn3.4504</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-7168-3257</orcidid><oa>free_for_read</oa></addata></record>
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subjects Acetic acid
acetic acid‐induced writhing
Alternative medicine
Analgesics
Anti-inflammatory agents
Antioxidants
Bioassays
Biopolymer denaturation
Blood levels
brine shrimp lethality bioassay
Caesalpinia digyna
Carrageenan
carrageenan‐induced paw edema
Cytotoxicity
Diabetes mellitus
DPPH assay
Edema
Erythrocytes
Free radicals
Herbal medicine
HRBC membrane lysis
Hypotonicity
Immunology
In vitro methods and tests
In vivo methods and tests
Inflammation
Leaves
Lethality
Lysis
Medicinal plants
Nonsteroidal anti-inflammatory drugs
Original
Phytochemicals
Plant extracts
Plants (botany)
Protein denaturation
Scavenging
Skin diseases
tail immersion
Vincristine
title Phytochemical Profiling and Pharmacological Evaluation of Methanolic Leaf Extract of C. digyna for Cytotoxic, Anti‐inflammatory, Antioxidant, Antiarthritic, and Analgesic Activities
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