Dominant-negative β1 integrin mice have region-specific myelin defects accompanied by alterations in MAPK activity

• Published in: Glia 2006-06, Vol.53 (8), p.836-844
• Main Authors: Lee, Karen K., De Repentigny, Yves, Saulnier, Ron, Rippstein, Peter, Macklin, Wendy B., Kothary, Rashmi
• Format: Article
• Language: English
• Subjects: AKT; Biological and medical sciences; cuprizone; Fundamental and applied biological sciences. Psychology; Isolated neuron and nerve. Neuroglia; MAP kinase; myelination; oligodendrocytes; PLP promoter; transgenic mice; Vertebrates: nervous system and sense organs
• ISSN: 0894-1491; 1098-1136
• DOI: 10.1002/glia.20343

Recent studies have demonstrated the importance of β1 integrin in oligodendrocyte maturation in vitro. Similar studies in vivo have been difficult due to the embryonic and perinatal lethality of null mutations in integrin subunits. Here, we have generated transgenic mouse models that overexpress full length β1 integrin or express a dominant‐negative β1 integrin ΔC (lacking the C‐terminal tail) under the control of the proteolipid protein (PLP) promoter. We demonstrate that these transgenes are expressed predominantly in CNS tissues and more specifically in oligodendrocytes. Further analysis reveals that the dominant‐negative β1 integrin ΔC transgenic mice, but not the full length β1 integrin mice, have hypomyelinated axons in spinal cords and optic nerves. In addition, there is a significant increase in the number of unmyelinated axons within the spinal cords and optic nerves of the β1 integrin ΔC mice. In contrast, the corpus callosum from these mice did not show similar myelin defects. To assess if remyelination would be affected in the corpus callosum, mice were subjected to a cuprizone‐induced demyelination. Interestingly, the dominant‐negative mice recovered from this insult in a manner similar to the wild type littermates. Axons within the corpus callosum that were remyelinated had normal g‐ratios; however, the actual percentage of myelinated axons was significantly reduced compared with wild type mice. We also show that the defects observed in the dominant‐negative β1 integrin ΔC mice are accompanied by disruption of the MAP‐kinase signaling pathway. Our work highlights the importance of β1 integrin‐mediated signaling in CNS myelination in vivo. © 2006 Wiley‐Liss, Inc.