E-cadherin abnormalities resulting from CPG methylation promoter in metastatic and nonmetastatic oral cancer

Background. This study aims to compare the alterations in the methylation profiles of E‐cadherin in oral cancer, especially in tumors with lowest metatastic potential. Methods. Nine oral verrucous carcinomas (VCs), 20 oral well‐differentiated squamous cell carcinomas without lymph node involvement (...

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Published in:Head & neck 2008-01, Vol.30 (1), p.85-92
Main Authors: de Moraes, Renato Vieira, Oliveira, Denise Tostes, Landman, Gilles, de Carvalho, Fabrício, Caballero, Otávia, Nonogaki, Suely, Nishimoto, Inês, Kowalski, Luis Paulo
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biological and medical sciences
Cadherins - genetics
Carcinoma, Squamous Cell - genetics
Carcinoma, Verrucous - genetics
CpG Islands - genetics
DNA Methylation
E-cadherin
Female
Humans
Immunohistochemistry
Lymphatic Metastasis
Male
Medical sciences
methylation
Middle Aged
Mouth Neoplasms - genetics
oral cancer
Otorhinolaryngology. Stomatology
Polymerase Chain Reaction
squamous cell carcinoma
Verrucous carcinoma
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Summary:Background. This study aims to compare the alterations in the methylation profiles of E‐cadherin in oral cancer, especially in tumors with lowest metatastic potential. Methods. Nine oral verrucous carcinomas (VCs), 20 oral well‐differentiated squamous cell carcinomas without lymph node involvement (SCC‐pN0), and 17 with lymph node involvement (SCC‐pN+) were analyzed using methylation‐specific polymerase chain reaction and immunohistochemical expression of E‐cadherin gene. Results. The immunohistochemical expression of E‐cadherin in VC was significantly higher (p = .016) when compared with SCC‐pN0 and SCC‐pN+ groups. The E‐cadherin gene methylation was not correlated with its abnormal immunohistochemical expression in VC and SCC‐pN0. All tumors of the SCC‐pN+ group with unmethylated E‐cadherin gene showed significant loss of E‐cadherin immunoexpression (p = .044). Conclusions. The E‐cadherin gene methylation presence in tumors with lowest invasive and metastatic potential, such as VC, suggests the early involvement of this epigenetic event in the multistep progression of the oral carcinogenesis. © 2007 Wiley Periodicals, Inc. Head Neck, 2008
ISSN:1043-3074
1097-0347
DOI:10.1002/hed.20666