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Penjweini et al. describe a new ‘sandwich’ probe comprised of two different fluorescent proteins that (via FRET and FLIM methods) read out the state of a myoglobin linked between them. The mCherry emission lifetime is primarily sensitive to oxygen binding, while the EYFP lifetime instead serves to r...

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Bibliographic Details
Published in:Journal of biophotonics 2022-03, Vol.15 (3)
Main Authors: Penjweini, Rozhin, Roarke, Branden, Alspaugh, Greg, Link, Katie A., Andreoni, Alessio, Mori, Mateus P., Hwang, Paul M., Sackett, Dan L., Knutson, Jay R.
Format: Article
Language:English
Online Access:Get full text
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Summary:Penjweini et al. describe a new ‘sandwich’ probe comprised of two different fluorescent proteins that (via FRET and FLIM methods) read out the state of a myoglobin linked between them. The mCherry emission lifetime is primarily sensitive to oxygen binding, while the EYFP lifetime instead serves to read out the met state of myoglobin. That latter state is a valuable indicator of ROS or NO exposure. Further details can be found in the article by R. Penjweini, B. Roarke, G. Alspaugh, K. A. Link, A. Andreoni, M. P. Mori, P. M. Hwang, D. L. Sackett, J. R. Knutson ( e202100166 ). image
ISSN:1864-063X
1864-0648
DOI:10.1002/jbio.202270003