A rapid estimation of prednisolone produced by fermentative oxidation

An analytical method has been devised for the rapid determination of prednisolone produced in a fermentative oxidation process. It allows frequent checking of concentrations of the prednisolone formed during the oxidation of hydrocortisone. The estimation of prednisolone in the presence of hydrocort...

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Bibliographic Details
Published in:Journal of biochemical and microbiological technology and engineering 1961-07, Vol.3 (2), p.119-123
Main Authors: Mizsei, Antal, Szabó, Antal
Format: Article
Language:English
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Summary:An analytical method has been devised for the rapid determination of prednisolone produced in a fermentative oxidation process. It allows frequent checking of concentrations of the prednisolone formed during the oxidation of hydrocortisone. The estimation of prednisolone in the presence of hydrocortisone is based upon the diversity of colour shown by the chromogens produced from these steroids by sulphuric acid and ethanol. Using the optimal reagent mixture and conditions, the interference of hydrocortisone is negligible. The steroids are extracted from the fermentation broth with ethyl acetate. One test takes about 20 min.
ISSN:0368-1467
1547-173X
DOI:10.1002/jbmte.390030202