Estradiol protects adipose tissue–derived stem cells against H 2 O 2 ‐induced toxicity

Oxidative stress is associated with various pathophysiological processes, including cell survival, adhesion, apoptosis, and cancer. In the present study, we aimed to evaluate the effects of H 2 O 2 ‐induced toxicity on adipose tissue–derived stem cells (ADSCs) and whether 17β‐estradiol (E 2 ) has pr...

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Published in:Journal of biochemical and molecular toxicology 2012-08, Vol.26 (8), p.301-307
Main Authors: Taskiran, Dilek, Evren, Vedat
Format: Article
Language:English
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Summary:Oxidative stress is associated with various pathophysiological processes, including cell survival, adhesion, apoptosis, and cancer. In the present study, we aimed to evaluate the effects of H 2 O 2 ‐induced toxicity on adipose tissue–derived stem cells (ADSCs) and whether 17β‐estradiol (E 2 ) has protective effects on these cells. ADSCs derived from adult Sprague–Dawley rats were pretreated with different doses of E 2 for 24 h and then exposed to 200 µM H 2 O 2 for 4 h. Incubation of ADSCs with H 2 O 2 ‐decreased cell viability in a concentration‐dependent fashion ( p < 0.0001), whereas pretreatment of these cells with E 2 significantly reversed toxicity ( p < 0.05), inhibited apoptotic changes, and decreased lipid peroxidation ( p < 0.0005). Our findings suggest that E 2 protects ADSCs from oxidative‐induced cell death, and therefore, it may be used to improve the survival rate and regenerative capacity of stem cells. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:301–307, 2012; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.21421
ISSN:1095-6670
1099-0461
DOI:10.1002/jbt.21421